To prove that a certain fruit (in this case, an orange) indeed contains a certain compound (in this case, vitamin C); then the vitamin C should be extracted and isolated from the orange and must be confirmed by molecular analysis. The extraction process involves using chemicals (i.e. 6% MPA), and procedures such as centrifugation and filtration. Then the extract is stored and subjected to high-performance liquid analysis (HPLC) to measure the vitamin C content.
Insertion mutations can be small, like in the CAGTC example in which only one nucleotide was inserted, or they can be large, with many nucleotides being added. If the number of bases inserted is not a multiple of three, then the insertion mutation is also considered a frameshift mutation.
Hope this helps :D