A Negative stain such as India ink or Congo red-Look for the presence of a capsule.
This stain method is usually used to stain the area around the microorganism.
B The Gram stain-Divide bacteria into two groups based on cell wall structure (thick vs. thin).
This is type of differential staining used to distinguish organisms based on their staining properties. Gram + and Gram- bacteria stain different because of different cell wall structure.
C The Ziehl-Neelsen Acid-fast stain- Identify bacteria with waxy cell walls such as: Mycobacterium tuberculosis (the cause of TB).
This is also differential staining method which uses heat and phenol to derive dye into the cells with lipid-rich walls.
D Simple stain with a basic dye-Stain microbes a bright color to make it easier to see them in bright field microscopy.
One dye is used in simple staining in order to determinate the size, shape and arrangement of the cells.
E The Schaeffer-Fulton Endospore stain-Identify Bacillus or Clostridium species, such as the causative agents of anthrax, botulism, tetanus and gangrene.
This is a special type of staining only used for the bacteria that can form endospores. Bacteria are first treated with heat and then with malachite green, which is very strong stain that can penetrate endospores.
Answer:
when G1/S cyclin-CDK is activated. Expression of G1/S cyclins is regulated by E2F proteins
Explanation:
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Commitment to cell division occurs at Start when G1/S cyclin-CDK is activated. Expression of G1/S cyclins is regulated by E2F proteins in conjunction with pRB proteins.. Mitogens stimulated cell division by increasing the amount of G1 cyclins.
Answer:
A. Molecular changes such as extracellular signals on extracellular ligands can result in quick changes in cell behaviour. One example is insulin. Upon insulin binding on the receptor on the cell membrane, the cell release GLUT4 transporters (for muscle cells) to increase uptake of glucose.
B. Slow changes in cell behaviour can be observed for other lipophilic hormones or intracellular receptors such as glucocoortoid or estrogen which reacts with receptors in the cells.
C. Insulin is required to maintain a constant blood glucose level and hence levels of insulin has to be mediated in correspondence to the blood glucose levels. Fast acting signalling is thus required for homeostasis of blood glucose levels. Where for such lipophilic hormones such as steriods, glucocortoid, these hormones tend to have a longer lasting effect and hence results in a slow change.
Domain is the most inclusive of the three, genus is the least inclusive
