Question

Martin is studying sucrase activity. He added sucrose solution + sucrase solution + buffer of pH 5.6 in two tubes. He incubated them at 35°C and 100°C respectively. When he performed a confirmatory test, only the first test tube showed enzyme activity. How would you justify the inactivity of the enzyme in the second test tube?

Answer 1

I'm pretty sure that this is the correct answer "The enzymes in the second test tube must have denatured at 1000C, since most enzymes degrade at such high temperatures." that is if you're using Plato, in which case this would be one of your answer choices.

Answer 2

Answer:

Enzymes require specific temperature to perform

Explanation:

Enzymes require specific temperature to  activate itself and catalyse a reaction. An enzyme which is able to perform well at a temperature of 35 degree Celsius will not be able to perform in the similar manner at a temperature of 100 degree Celsius or vice Versa. An enzyme that is meant to get activated at lower temperature will get denatured at high temperature and thus will not be able to carry out its activity. While on the other hand an enzyme that  is meant to  operate at high temperature will not be able to get into activation mode at lower temperature.