Answer:
Background
During the course of a bacterial infection, the rapid identification of the causative agent(s) is necessary for the determination of effective treatment options. We have developed a method based on a modified broad-range PCR and an oligonucleotide microarray for the simultaneous detection and identification of 12 bacterial pathogens at the species level. The broad-range PCR primer mixture was designed using conserved regions of the bacterial topoisomerase genes gyrB and parE. The primer design allowed the use of a novel DNA amplification method, which produced labeled, single-stranded DNA suitable for microarray hybridization. The probes on the microarray were designed from the alignments of species- or genus-specific variable regions of the gyrB and parE genes flanked by the primers. We included mecA-specific primers and probes in the same assay to indicate the presence of methicillin resistance in the bacterial species. The feasibility of this assay in routine diagnostic testing was evaluated using 146 blood culture positive and 40 blood culture negative samples.
Explanation:
Results
Comparison of our results with those of a conventional culture-based method revealed a sensitivity of 96% (initial sensitivity of 82%) and specificity of 98%. Furthermore, only one cross-reaction was observed upon investigating 102 culture isolates from 70 untargeted bacteria. The total assay time was only three hours, including the time required for the DNA extraction, PCR and microarray steps in sequence.
Answer:
B. Guyot
Explanation:
What are guyots?
- Guyots are very similar to seamounts, but they have a flat surface on the top rather than a pointy one. The image shown on the question is a guyot, as it's a seamount with a flat top.
What are seamounts?
- Seamounts are basically underwater mountains on the ocean floor.
Have a lovely rest of your day/night, and good luck with your assignments! ♡
Geothermal energy comes from the heat within the earth. The word "geothermal" comes from the Greek words geo, meaning earth," and therme, meaning "heat."
Answer:
Flowers and cones have different structures. Cones when mature have a woody texture and is made up of scales while flowers have colorful petals, sepals and both male and female reproductive portions are called stamens and pistils.
Explanation:
I believe this is T. Because i just learned that in science not that long ago, and deciduous forests have small winters and long growing seasons, and the small winters have lots of snow, and the long growing seasons have lots of rain.
