Nutrients feels like the right word... hope that helps
The answer is Ordovician-Silurian.
The mass extinction thaw was most likely caused by the extinction that was most likely caused by the formation and retreat of glacier was the Ordovician-Silurian extinction. It is the second largest among the five major extinction events.
Plants have large Vacuoles because they keep their water stored inside. Since plants live almost entirely off of water, their vacuoles are larger unlike those in an animal cell.
Contaminating ground water, pollution, exposure to toxic chemicals