Answer:
is bound to the constant region of the secondary antibody.
Explanation:
Enzyme immunoassays are the techniques used to detect the presence of antigens with the help of antibodies. Each of the antibody molecules has a constant and variable region.
The primary antibodies are added to the wells. The constant region of the secondary antibody is bound to an enzyme while its variable region is free so that it can bind to the specific antigen.
Addition of substrate to the system is followed by visualization and/or evaluation of antigen as the reaction between enzyme and substrate produce some visible changes such as color change.
Answer:
Sarcomere
Explanation:
A sarcomere is the basic contractile unit of muscle fiber. Each sarcomere is composed of two main protein filaments—actin and myosin—which are the active structures responsible for muscular contraction.
Answer:
Background
During the course of a bacterial infection, the rapid identification of the causative agent(s) is necessary for the determination of effective treatment options. We have developed a method based on a modified broad-range PCR and an oligonucleotide microarray for the simultaneous detection and identification of 12 bacterial pathogens at the species level. The broad-range PCR primer mixture was designed using conserved regions of the bacterial topoisomerase genes gyrB and parE. The primer design allowed the use of a novel DNA amplification method, which produced labeled, single-stranded DNA suitable for microarray hybridization. The probes on the microarray were designed from the alignments of species- or genus-specific variable regions of the gyrB and parE genes flanked by the primers. We included mecA-specific primers and probes in the same assay to indicate the presence of methicillin resistance in the bacterial species. The feasibility of this assay in routine diagnostic testing was evaluated using 146 blood culture positive and 40 blood culture negative samples.
Explanation:
Results
Comparison of our results with those of a conventional culture-based method revealed a sensitivity of 96% (initial sensitivity of 82%) and specificity of 98%. Furthermore, only one cross-reaction was observed upon investigating 102 culture isolates from 70 untargeted bacteria. The total assay time was only three hours, including the time required for the DNA extraction, PCR and microarray steps in sequence.
The most abundant element is oxygen...found in the crust and in the atmosphere.
Genetic defects can be cause by any chromosome, whether an autosome or a sex chromosome.
