Answer:
Explanation:
The eukaryotic genes of interest can be expressed in the prokaryotic genome or plasmid vector. The following are the steps used for the purpose of expression of eukaryotic genes in the prokaryotic genome.
1. Amplification of the target gene of interest from the eukaryotic genome by PCR or polymerase chain reaction.
2. The selection of the plasmid and digestion of the plasmid DNA with the restriction enzyme.
3. Ligation of the amplified DNA fragment and the plasmid.
4. Introduction of the ligated plasmid into the prokaryotic host organism.
Answer:
Distractions is a common mistake
Explanation:
Answer:
Following are the responses to the given points:
Explanation:
In point a:
Its reaction provided is indeed a type of radioactive substitution reaction. Its fluoride group (F group) is a poor leaving group, in which carbon atoms attached to the carbon atom reducing their energy. Thus fluoride group replacement becomes complicated again for the thiol group.
In point b:
When fluoroacetamide is blended with cystine, they associate due to the proximity of ligands, resulting in the formation of a complex of hydrogen bond proteins.
