Answer:
the diagram explains the process of DNA digestion and DNA ligation, which is usually used in molecular cloning techniques
Explanation:
Molecular cloning can be defined as the process used to synthesize multiple copies of a particular DNA fragment. Molecular cloning requires the insertion of a foreign DNA fragment into an appropriate vector (e.g., a plasmid) through the action of specific enzymes that serve to cut and ligate DNA fragments. DNA digestion and DNA ligation use specific restriction enzymes and DNA ligases, respectively, in order to insert the foreign DNA fragment. For this purpose, restriction enzymes that generate single-stranded overhangs are preferred to create sticky ends which bind by complementary base pairing. Subsequently, a DNA ligase enzyme joins the DNA fragments together in order to create recombinant DNA molecules. DNA Ligation is often achieved by using a specific T4 DNA ligase, while there are many restriction enzymes that generate sticky-ends (e.g., BamHI, EcoRI, BaI228I, etc).
Answer:
b. Frank received the mutant chromosome from his father. Nondisjunction occurred in his father during the first meiotic division.
Explanation:
As you can see in the question above, Frank has Klinefelter syndrome which causes him to have normal skin patches and skin patches without sweat glands. Her mother has completely normal hair, which may indicate that the defective gene was not supplied by her. In addition, Frank's father has anhydrotic ectodermal dysplasia, an X-linked condition where the skin does not contain sweat glands.
Although Frank's father's defective gene is linked to the X chromosome, it is likely that Frank inherited the defective gene from his country. This may have occurred because during meiosis I, his father's genes did not show disjunction. As a result, Frank presents a mosaic of his phenotype, because an inactivation of the X chromosome occurred.
A nonfunctioning protein will be produced
The Hershey–Chase experiment was based on a bacteriophage T2 (a virus), to that DNA is the genetic material. Bacteriophage T2 attacks bacterium and makes its copies. Based on the experiment, Hershey and Chase deduced that it is the DNA of virus enters bacteria to make virus copies. To trace viral DNA, Hershey and Chase label DNA with ³²P (radioisotope of phosphorus) because phosphorus is not present in most of the proteins.
