Question

A lab protocol asks you to place 1 gram of tissue into 9 ml of PBS and then homogenize the mixture. You are then asked to take 1 ml of that homogenate and add 9 mls of PBS. Using a standard curve, you determine that the diluted mixture contains 10 micrograms of protein per mL. What is the concentration of protein in the homogenate ?

Answer 1

Answer:

100 micrograms per mL.

Explanation:

The PBS diluted mixture used for the standard curve measurement of protein concentration is a 1:10 dilution from the original homogenate of the protein with PBS. So, if the diluted mixture contains 10 micrograms per mL, the original homogenate contains 10x that. 10*10= 100 micrograms per mL.