Question

Intracellular condensates are non-membrane bound biochemical subcompartments that form due to phase separation among networks of weakly interacting molecules. Sabari et al., 2018, proposed that the transcriptional coactivator BRD4 helps form intracellular condensates containing other transcriptional proteins. A prediction of this proposal is that BRD4 should behave as a liquid within the condensate with rapid movement. Which procedure could be used to analyze movement of BRD2 in living cells?

Answer 1

Answer:

Fluorescence recovery after photo bleaching.

Explanation:

This is a very valuable microscopic technique in cell biological research which is process used to measure molecular diffusion and transport. This process is based on irreversible bleaching of pool fluorescent probes in order to monitor the recovery in fluorescence caused by the movement of intact probes into the bleached spot.