Question

You transform an E. coli bacterium with the pGLO plasmid. After growing the +pGLO bacteria on an LB+Ampicillin+Arabinose plate, you see distinct colonies form after 16 hours. You check these colonies under the UV light and see that they are glowing a bright fluorescent green color. If you use a sterile loop and inoculate a new plate with LB+Ampicillin+Arabinose (coated on them, as usual) with these transformed E. coli will you see any growth? If there is growth, will it glow?A. Yes, we will see growth. Yes, they will glow. B. Yes, we will see growth. No, they will not glow. C. No, we will not see any growth. No, they will not glow. D. No, we will not see any growth. Yes, they will glow.

Answer 1

Answer:

Option A: Yes, we will see growth, and Yes, they will glow

Explanation:

The pGLO plasmid has very genetical determinants, particularly a gene coding for a Beta-lactamase, that provides this bacteria resistance to B-lactam antibiotics.  It also has a gene coding for the green fluorescent protein (GFP), responsible for green glowing fluorescence.  These are called repirter genes.

A coding gene to metabolize arabinose is also present in the plasmid, with a promoter also used for the expression of GFP when arabinose is present on the plate.

Transformed E. coli (with pGLO plasmid) cells will grow on a plate containing ampicillin, and their colonies will glow when arabinose is also present.