Answer:
b. A second marker in the knock-out cassette, that if inserted into the genome results in cell death when plated on selective media.
Explanation:
General recombination, also known as homologous recombination, refers to the naturally occurring process of exchange of genetic material between pairs of homologous DNA sequences. This process (homologous recombination) can be exploited by genetic engineering to insert DNA segments of interest at target genes. Moreover, a cassette is a mobile DNA segment containing almost a gene and a recombination site, which is integrated into the <em>locus</em>/<em>loci</em> of interest by homologous recombination. A cassette may contain a DNA segment called 'negative marker' which prevents growth under particular conditions, while a positive marker permits growth under certain conditions. In consequence, a second marker consisting of a drug cassette may be used as a negative marker in order to evidence its insertion by inducing cell death when they are plated in selective conditions.
Answer: option D) phylum
Explanation:
The linnaean system of classification from largest to smallest level is as follows:
Kingdom ---> phylum ---> class ---> order ---> family ---> genus ---> specie.
Since the phylum level consists of organisms with less similar characteristics compared to class, order and genus, we can safely say it has most organisms.
Thus, phylum is the answer.
<span>B) The volcanic gases released from the deep sea vents are similar to those released into the early atmosphere.</span>
Glycolysis is the process responsible for generating
the most ATP within cellular respiration
<span>
During cellular respiration, the
food molecules such as glucose, are oxidized to carbon dioxide (CO2) and water (H2O)
and trapped in ATP (Adenosine triphosphate) form for further us of cell’s
activities. ATP’s are formed at mitochondria – the cell’s powerhouse. This type
of organelle takes and breaks nutrients absorbed by the cell and creates energy
afterward. The energy from ATP is then used by the body in kinetic activities
like running & walking or involuntary activities like breathing, blood
circulation, stimulus-responding, etc.</span>
