Answer:
Koch's postulates say that microbes are present in the case of every disease and these disease-causing microbes can be isolated to grow in pure culture. He also said that this pure culture if transferred in healthy individual it will cause disease.
Chytrids contain many parasite fungus that infects amphibians and by using Koch postulate we can show that chytrid infections are responsible for the frog deaths.
First, we have to take the sample from dead diseased amphibian then this sample should be inoculated in a selective media which supports the growth of chytrid fungus and not bacteria.
Then this pure culture of fungus is transferred in a healthy amphibian. The death from chytrid infection of healthy amphibian will show that chytrid infections are responsible for the frog deaths.
Answer:
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Answer:
- DNA to be cloned
- mature mammalian mRNA is isolated
- reverse transcriptase, provided with a primer, synthesizes a strand of cDNA without introns
- the mRNA is hydrolyzed by raising the pH
- a primer site is created on the ssDNA
- a complementary DNA strand is synthesized, forming a ds cDNA molecule.
- dsDNA
Explanation:
cDNA is the acronym for complementary DNA. This DNA is a single or double stranded moolecule that is synthesized from a messenger RNA molecule. This molecule is used in studies that aim to clone eukaryotic genes in prokaryotic individuals. The result of this is the expression of a protein from a eukaryotic individual in prokaryotic individuals.
To form a double-stranded cDNA molecule, the following steps would be required:
- DNA to be cloned
- mature mammalian mRNA is isolated
- reverse transcriptase, provided with a primer, synthesizes a strand of cDNA without introns
- the mRNA is hydrolyzed by raising the pH
- a primer site is created on the ssDNA
- a complementary DNA strand is synthesized, forming a ds cDNA molecule.
- dsDNA
Answer: Largest to smallest: Nucleus, Chromosome, Gene
Explanation:
Nucleus is the big shell that holds all the chromosomes
Chromosome is in the nucleus
A gene is part of the chromosome
It’s see most of the interior of each cell is occupied
