Afterbirth involves the expulsion of placenta, umbilical cord and fetal membranes from the uterus followed by the birth of child. While the fluid from the amniotic sac is released before or during the labor which is also called water breaking.
There are two main variables in an experiment: the independent and dependent variable.
The experimenter changes the independent variable and that has the influence on dependent variable. So, the effect on the dependent variable is tested (observed and recorded).