Transcription and splicing only occur in eukaryotic cells. Transcription and splicing both occur in the nucleus.
Explanation:
Transcription is mediated by RNA polymerases that transcribe the DNA into RNA. In eukaryotes, because coding regions (exons) on the DNA are occasionally interspersed with non-coding regions (introns), the formed RNA (usually called nascent RNA) need to be spliced to remove the non-coding parts. This forms a mature messenger-RNA. All this occurs in the nucleus of eukaryotic cells and only after the mature mRNA is formed is it exported to the cytosol for translation into proteins by ribosomes.
Splicing does not occur in prokaryotic cells because their genome is all composed of coding regions. Therefore transcription on DNA produces, directly, a mature mRNA.
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