Answer:
Extraction of genomic DNA
Explanation:
Resuspend the cells(any number), smaller numbers also work, in 10-20 ul of TE buffer or milli Q water. boil for 5-10 min in a waterbath, quick chill, short spin. use the supernatant as a template for PCR.
If cells are very less, u can directly add cells to the PCR reaction without enzyme,boil for 5-10 min in a waterbath, let it cool slowly, short spin. Add enzyme to the supernatant and start the reaction at extension step followed by normal PCR reaction steps.
Macronutrients are nutrients that provide calories or energy. Nutrients are substances needed for growth, metabolism, and for other body functions. Since “macro” means large, macronutrients are nutrients needed in large amounts. There are three macronutrients:CarbohydrateProteinFatso its not B
There are three<span> primary types of </span>antioxidants<span> found in nature. These include phytochemicals, vitamins, and enzymes.
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So not C.
<span>The three micronutrients are vitamins, minerals, and water.
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So not A.
And that leaves you with D.(D is the correct answer for num.1)
Answer:
Human activities have a tremendous impact on the carbon cycle. Burning fossil fuels, changing land use, and using limestone to make concrete all transfer significant quantities of carbon into the atmosphere
1. Pedigrees
2. Uppercase
Lowercase