Cholinergic neurons release acetylcholine as a neurotransmitter. The cell bodies of preganglionic neurons of sympathetic division are present in the lateral horns of 12 thoracic and first two lumbar segments of the spinal cord. These are the cholinergic neurons and their axons release the acetylcholine as neurotransmitter into the synaptic cleft. The acetylcholine released by preganglionic sympathetic neurons binds to the cholinergic receptors present in the membranes of postganglionic neurons of the sympathetic division.
