<h2>Density dependent </h2>
Explanation:
Density dependent inhibition is the process exhibited by most normal (anchorage dependent) animal cells in culture that stop dividing once a critical cell density is reached
The critical density is considerably higher for most cells than the density at which a mono-layer is formed
As cells become more numerous, the cell surface proteins of one cell contact the adjoining cells and they signal each other to stop dividing
Normal cells proliferate until they reach finite density but cancer cells are not sensitive to density dependent inhibition
Answer:
See below
Step-by-step explanation:
All the enzymes used in the glycolysis reactions are proteins.
The body can use proteins as alternate sources of energy, but they are involved only in the Tricarboxylic Acid Cycle and the reactions linking glycolysis to the cycle, and these occur after glycolysis.
Answer:
tetracycline
Explanation:
When the DNA insert is ligated in Pstl site, the ampicillin gene is disrupted while <em>tetracycline gene is still intact</em>.
- If the bacterial colony is growth in a medium with only ampicillin, only the cells that contains the plasmid with the insert will grow.
- If the bacterial colony is growth in a medium with only tetracycline, the cells which have incorporated the plasmid will survive. We cannot distinguish between cells with plasmid + insert and plasmid alone.
- If the bacterial colony is growth in a plate with ampicillin + tetracyclin, only the cells with the plasmid without the insert will grow.
Therefore, to ensure that the cells of a bacterial colony contains the plasmid, they have to be seeded in a plate with tetracycline.
