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xxMikexx [17]
3 years ago
10

Help me out anybody plz

Chemistry
1 answer:
Sergio039 [100]3 years ago
7 0
The answer would be the third one
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Methods: Part A: Preparation of Buffers Make two buffers starting with solid material, which is the most common way to make buff
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Answer:

0,542 g of Na₂HPO₄ and 0,741 g of NaH₂PO₄.

0,856 g of Tris-HCl and 0,553 g of Tris-base

Explanation:

It is possible to use Henderson–Hasselbalch equation to estimate pH in a buffer solution:

pH = pka + log₁₀ \frac{A^{-} }{HA}

Where A⁻ is conjugate base and HA is conjugate acid

The equilibrium of phosphate buffer is:

H₂PO₄⁻ ⇄ HPO4²⁻ + H⁺    Kₐ₂ = 6,20x10⁻⁸; <em>pka=7,21</em>

Thus, Henderson–Hasselbalch equation for phosphate buffer is:

pH = 7,21 + log₁₀ \frac{HPO4^{2-} }{H2PO4^{-} }

If desire pH is 7,0 you will obtain:

<em>0,617 =  \frac{HPO4^{2-} }{H2PO4^{-} } </em><em>(1)</em>

Then, if desire concentration of buffers is 0,10 M:

0,10 M = [HPO₄²⁻] + [H₂PO₄⁻] <em>(2)</em>

Replacing (1) in (2) you will obtain:

<em>[H₂PO₄⁻] = 0,0618 M</em>

And with this value:

<em>[HPO₄²⁻] = 0,0382 M</em>

As desire volume is 100mL -0,1L- the weight of both Na₂HPO₄ and NaH₂PO₄ is:

Na₂HPO₄ = 0,1 L× \frac{0,0382mol}{1L}× \frac{141,96g}{1mol} = 0,542 g of Na₂HPO₄

NaH₂PO₄ = 0,1 L× \frac{0,0618mol}{1L}× \frac{119,96g}{1mol} = 0,741 g of NaH₂PO₄

For tris buffer the equilibrium is:

Tris-base + H⁺ ⇄ Tris-H⁺ pka = 8,075

Henderson–Hasselbalch equation for tris buffer is:

pH = 8,075 + log₁₀ \frac{Tris-base }{Tris-H^{+} }

If desire pH is 8,0 you will obtain:

<em>0,841 =  \frac{Tris-base }{TrisH^{+} } </em><em>(3)</em>

Then, if desire concentration of buffers is 0,10 M:

0,10 M = [Tris-base] + [Tris-H⁺] <em>(4)</em>

Replacing (3) in (4) you will obtain:

[Tris-HCl] = 0,0543 M

[Tris-base] = 0,0457 M

As desire volume is 100mL -0,1L- the weight of both Tris-base and Tris-HCl is:

Tris-base = 0,1 L× \frac{0,0457mol}{1L}× \frac{121,1g}{1mol} = 0,553 g of Tris-base

Tris-HCl = 0,1 L× \frac{0,0543mol}{1L}× \frac{157,6g}{1mol} = 0,856 g of Tris-HCl

I hope it helps!

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