Answer:
horse and donkey.
Explanation:
their bodies are similar. they also sound the same
This is what is commonly know as 'peat'. Peat is found in peatlands, bogs, mires and moors, and is an accumulation of partially decayed vegetation or organic matter. The decay is slowed down by a lack of oxygen due to waterlogged conditions. These areas are very important carbon sinks as the CO2 released by the decaying matter is trapped within the peat. It requires thousands of years for peatland to develop. Peatlands are very important as they provide a record of past vegetation and climate within the preserved plant remains.
Answer:
aorta
Explanation:
The aorta and its branches carries the blood to all the body's tissues.
Sink to the bottom because it is more dense than the water.
Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
