The role that the mosquito played is that the mosquito was carrying the organism/infection so once it bit Jane, Jane got infected. (Not sure is that’s what your asking but that’s the best I can explain it.)
Self esteem increases during adulthood because you develop self esteem through experiences and learning. This develops confidence which is correlated to high self esteem.
A healthy weight, is a set BMI (Body mass index) for your age. The doctor uses a BMI chart to determine the best weight for you based on your height and age.