The role that the mosquito played is that the mosquito was carrying the organism/infection so once it bit Jane, Jane got infected. (Not sure is that’s what your asking but that’s the best I can explain it.)
Puncture wounds would be when something punctures the skin, i. e., I stepped on a nail, giving me a puncture wound. When the skin scrapes against a hard surface and tiny blood vessels break in the outer layer of skin, this is called an "abrasion"
