Stars begin to form from clouds of gas in space. The cold temperatures and high densities (compared to elsewhere in space, but would be considered a vacuum on Earth) of these clouds allow gravity to overcome thermal pressure and start the gravitational collapse that will form a star.
A. Water being pumped in and out of guard cells.
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During the S phase in the cell cycle, the chromosomes are replicated so that there are two copies. These copies are necessary so that each daughter cell created during the M phase will have an identical copy of the DNA.
Therefore, the answer is A: The DNA must be replicated.
The chromosomes joining, cell membrane expanding, and cytoplasm separating all happen during the M phase (mitosis).
