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AnnyKZ [126]
3 years ago
12

A fourth type of restriction enzyme is identified that cleaves at precise locations like a type II enzyme, but it also occasiona

lly makes mistakes and cuts elsewhere. Would this type of enzyme be a useful enzyme to use in molecular cloning?
Biology
1 answer:
Mkey [24]3 years ago
6 0

Answer:

No, in molecular cloning cutting mistakes can lead to in correct ligation

C) No, because cutting mistakes could lead to incorrect cloning.

Explanation:

in molecular cloning, if you want to insert a specific gene of interest in to specific vector, the selection of enzyme and strategy before that is very important.  The restriction enzyme should be selected which restrict at the desired location in the cloning sites. Any mistake in these selection or undesirable mutation mistakes can lead to in correct or unsuccessful cloning results.  

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Which specialized cells are responsible for transmitting messages throughout the body
FrozenT [24]
The answer is Neurons. Good luck
4 0
3 years ago
In a separate maddyase experiment using (Et)-10nM, the reaction velocity is measured as 3uMS. What is the substrate concentratio
Vinvika [58]

Answer: hello your question has some missing data attached below is the missing data

Applying the Michaelis-Menten Equation

An enzyme is found that catalyzes the reaction X ⇋ Y Researchers find that kcat for this enzyme is 600s^-1 and carry out several additional experiments.

answer :

substrate concentration [ x ] = 10⁻⁵ M

Explanation:

Given data:

[Et ] = 10 * 10⁻⁹ M ,  

Reaction velocity ( V₀ ) = 3* 10⁻⁶ Ms⁻¹

applying Michaelis-Menten  Equation

= 3* 10⁻⁶ = \frac{600 *10*10^{-9} * (X)  }{10^{-5} + (X) }  ,    

= ( 3 * 10⁻⁶ )( x ) + ( 3 * 10⁻¹¹ ) = ( 6 * 10⁻⁶ ) ( x )

hence the substrate concentration [ x ] = 10⁻⁵ M

5 0
3 years ago
Points in the cell cycle where the cell "looks" for errors are called what
Nonamiya [84]

Answer:

The correct answer is - checkpoints.

Explanation:

In the cell cycles, there are different stages, and before every stage are monitored by the internal controls known as checkpoints. These checkpoints monitored the errors. Checkpoints are present in 1st the end of G1, a 2nd at the G2/M transition, and the 3rd during the metaphase of the cell cycle.

The most important checkpoint is at the end of G1 which decides that cell will go in the division or not.

5 0
3 years ago
If the bacteria E.coli synthesize DNA at a rate of 100,000 nucleotides per min and takes 30 min to replicate the DNA how many ba
algol13
Based just on the information given, 1.5 million bp. 


 



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Question 23
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