Question

In a separate maddyase experiment using (Et)-10nM, the reaction velocity is measured as 3uMS. What is the substrate concentration used in the experiment

Answer 1

Answer: hello your question has some missing data attached below is the missing data

Applying the Michaelis-Menten Equation

An enzyme is found that catalyzes the reaction X ⇋ Y Researchers find that kcat for this enzyme is 600s^-1 and carry out several additional experiments.

answer :

substrate concentration [ x ] = 10⁻⁵ M

Explanation:

Given data:

[Et ] = 10 * 10⁻⁹ M ,  

Reaction velocity ( V₀ ) = 3* 10⁻⁶ Ms⁻¹

applying Michaelis-Menten  Equation

= 3* 10⁻⁶ = $\frac{600 *10*10^{-9} * (X) }{10^{-5} + (X) }$  ,    

= ( 3 * 10⁻⁶ )( x ) + ( 3 * 10⁻¹¹ ) = ( 6 * 10⁻⁶ ) ( x )

hence the substrate concentration [ x ] = 10⁻⁵ M