In a separate maddyase experiment using (Et)-10nM, the reaction velocity is measured as 3uMS. What is the substrate concentratio
1 answer:
Answer: hello your question has some missing data attached below is the missing data
Applying the Michaelis-Menten Equation
An enzyme is found that catalyzes the reaction X ⇋ Y Researchers find that kcat for this enzyme is 600s^-1 and carry out several additional experiments.
answer :
substrate concentration [ x ] = 10⁻⁵ M
Explanation:
Given data:
[Et ] = 10 * 10⁻⁹ M ,
Reaction velocity ( V₀ ) = 3* 10⁻⁶ Ms⁻¹
applying Michaelis-Menten Equation
= 3* 10⁻⁶ = ,
= ( 3 * 10⁻⁶ )( x ) + ( 3 * 10⁻¹¹ ) = ( 6 * 10⁻⁶ ) ( x )
hence the substrate concentration [ x ] = 10⁻⁵ M
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