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svetoff [14.1K]
4 years ago
14

When performing column chromatography to purify a protein of interest, one needs an assay to identify which fractions that conta

in the protein of interest. True or False: As discussed in lecture, we use a different assay for each different protein that we purify (For instance, the assay for protein A will be different than the assay for protein B which will be different than the assay for protein C, etc).
Biology
1 answer:
Mumz [18]4 years ago
7 0

Answer:

True

Explanation:

Protein purification involves isolating them from their source based on differences in their physical properties. The objective in chromatography is to retain the largest amount of functional protein with the least number of contaminants, which is why an assay needs to be performed for each type of protein.

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Histones are essentially identical in sequence/structure in all eukaryotic organisms from yeast to plants to animals. What does
xxTIMURxx [149]

Answer:

It indicates that core histone genes were present in the last common ancestor of yeasts, plants, and animals

Explanation:

Histones are highly basic proteins that can strongly interact with DNA, which is packaged into nucleosomes, the basic structural and functional unit of chromatin. Each nucleosome is composed of approximately 147 base pairs of DNA wrapped around a core of eight histone proteins (two copies of four types of histones H3, H4, H2A, H2B). These core histones are evolutionarily conserved across eukaryotic kingdoms in terms of sequence and structure. Therefore, DNA-packaging into nucleosomes is considered a constraint for the evolution of core histones. Moreover, the presence of conserved core histones in eukaryotic kingdoms (e.g., yeast, plant, and animal kingdoms) is strong evidence that histone-mediated DNA packaging was presumably present in the last common ancestor of eukaryotic genomes.

5 0
3 years ago
Know what makes a solution acidic or basic (the pH number and the ions).
Brrunno [24]

To determine whether an acidic or basic solution, it is first necessary to compare the concentrations of the hydronium (H3O +) and hydroxide (OH-) ions in the solution.

In acidic solution, the concentration of H3O + ions is higher than that of OH- ions.

  • In acidic solution, the concentration of H3O + ions is higher than that of OH- ions. Such a solution can be achieved by adding a small part of the H3O + ions, for example. Acid solutions have a pH below 7, the further away from 7 the pH of the solution is the higher its acidity content.  According to Le Chatelier's principle, when a disturbance is caused to an equilibrium system, it tends to readjust in order to diminish the effects of that force. This means that if an acid is added to water, the H3O + ions will be in excess and the equilibrium will shift in the opposite direction to the left. Then these excess ions will react with the OH- ions. Thus, the concentration of OH- ions will decrease and the solution will become acidic.
  • In basic solutions, the concentration of OH- ions is higher than that of H3O + ions.  If we add a base to the water, it means that we will be adding OH- ions and, as explained in the previous section, by Le Chatelier's principle, the equilibrium of the water selfionization reaction will shift in the opposite direction, and the excess ions will react. with the H3O + ions, decreasing their concentration and making the basic solution.  Basic solutions have a pH greater than 7, the farther from 7 and closer to 14 the pH of the solution, the higher the basification content.

8 0
3 years ago
Which one is the the oldest and youngest?​
patriot [66]

Answer:

The oldest is G

Explanation:

the youngest is B

3 0
3 years ago
Read 2 more answers
The chaparral biome is best characterized by _______. a. large, contiguous regions of shrubland b. small regions interspersed wi
Alinara [238K]
The correct answer should be B. Small regions interspersed with other biomes. This type of a biome is found on all continents and in many countries. It's specific because it's similar to a desert when it comes to yearly precipitation, but it is different insofar that it has a lot of plants and animals that are mostly nocturnal predators.
7 0
3 years ago
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Which of the three major groupings of marine life (plankton, nekton, benthos) could live without water?
Igoryamba
I think benthos they are like shells

7 0
3 years ago
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