Gram's staining is a differential staining technique that employs a primary stain like crystal violet and a counter stain like safranin along with the decolourizing agent alcohol and a mordant called the Gram's iodine.
Iodine is a mordant added after the primary stain. It fixes the stain by combining with it to enchance the staining ability. This forms an insoluble crystal violet iodine complex appearing purple under the microscope. These microorganisms are classified as Gram positive.
If addition of iodine is skipped, crystal violet is not fixed on the slide and the insoluble complex is not formed. The cells are decolourized by alcohol and are stained by the counter stain safranin making the Gram positive cells wrongly indentified as Gram negative due to its pink colouration. Thus, the slide will show all the cells as pink coloured Gram negative cells.
1.It is a universal solvent.
2Pure water is transparent, colourless and odourless.
3.Pure water is a poor conductor of electricity.
4.Water in a pure form has a neutral ph i.e 7 which means it is neither acidic nor basic.
An hypothesis is an educated guess whereas a theory has supporting evidence to help prove the point to be correct. (I THINK this is right)
Answer:
I believe it is Protein.
Explanation:
Hope my answer has helped you!
1. Cells
2. Tissues
3. Organs
4. Organ systems
5. Organisms
I hope this helped ^^
