Release factors will be bound to the A site of the ribosomes when the stop codon appear
Explanation:
The stop codon are not recognizable by tRNA so Release factor 1 will recognize UAA and UAG stop codons or Release factor 2 will recognize UAA and UGA stop codon, gets bind to the A site of ribosomes and triggers the hydrolysis of ester bond in peptidyl tRNA (between peptide and tRNA) and facilitates the release of newly synthesized peptide chain from the ribosomal assembly. Later on Release Factor 3 will catalyzes the removal of Release factor either 1 or 2 depending on the type of codon present on mRNA from the ribosomal assembly.
