Answer:
option A
Explanation:
In carrying out these experimental procedure, the same restriction enzyme that is used to cut out the gene of interest should also be the same used for the cutting of the plasmid to generate sticky ends. Then the desired gene is inserted into the cut plasmid then treatment with DNA ligase. Purified DNA ligase is utilized in gene cloning to join/ligate DNA molecules together forming a recombinant DNA. Thus, step 3 should be carried out after step 4.
The competitive exclusion principle states that two species cannot occupy the same niche. When two species compete for the same habitat and resources, the end result is that one species eliminates the other.
Answer:
Nitrogenous;Acid-Base and Fluid
Explanation:
The kidney is referred to as an excretory organ because it excretes nitrogenous wastes. It is also a major homeostatic organ because it maintains the electrolyte Acid-Base, and fluid balance of the blood.
