Question

You are doing research in bacteria on the effects of mutating the shine-dalgarno sequence. you change some nucleotides in the shine-dalgarno sequence and find that translation is greatly reduced. what other element in the bacteria could you change that would have a good chance of restoring translation in the bacterial strain with the altered shine-dalgarno sequence?

Answer 1

Shine-Dalgarno sequence is a sequence that precedes the starting codon in the mRNA. the small sub-unity of ribosome will recognize it with by a specific sequence in the initiation phase of translation.

The best way to restore the translation speed if the Shine Dalgarno sequence is mutated is to make a change in the small sub-unity of the ribosome, specifically in the RNA sequence that recognizes the mRNA Shine Dalgarno sequence, so it can be recognized again. and the translation process will be restored.
Another way to improve a translation speed if to provoke a mutation in the proteins' genes involved in the translation process (like enzymes and translation factors), there will be a chance to increase their effectiveness, especially for enzymes, so the translation speed will be improved.

Answer 2

The most ideal approach to reestablish the interpretation speed if the Shine Dalgarno sequence is transformed is to roll out an improvement in the little sub-solidarity of the ribosome, explicitly in the RNA arrangement that perceives the mRNA Shine Dalgarno succession, so it very well may be perceived once more. what's more, the interpretation procedure will be reestablished.  

Another approach to improve an interpretation speed if to incite a change in the proteins' qualities engaged with the interpretation procedure (like catalysts and interpretation factors), there will be an opportunity to expand their viability, particularly for compounds, so the interpretation speed will be improved.

Further details

Shine–Dalgarno sequence

It is a sequence having five to nine (regularly seven) nucleotides going before the start codon in prokaryotic mRNA that is perceived by the ribosome as the right site for restricting the mRNA atom preceding the beginning of interpretation. AGGAGGU binds a complementary sequence on the 16S ribosomal subunit, framing a steady mind boggling between the ribosome and mRNA. The job of this grouping was first proposed by Lynn Dalgarno 1935 and John Shine 1946.

Role of Shine-Dalgarno sequence in translation

It plays vital role in translation. In prokaryotic frameworks, the interpretation commencement of many, however not all, mRNAs rely upon collaboration between an arrangement component upstream of the start codon (the Shine-Dalgarno grouping [SD]) and a corresponding succession in the 3′ end of 16s rRNA [aSD]. Numerous chloroplast mRNAs harbor putative SDs in their 5′ untranslated districts and the aSD shows solid preservation, the useful importance of SD-aSD cooperations in plastid interpretation is hazy. Here, by creating transplastomic tobacco (Nicotiana tabacum) freaks with point transformations in the aSD combined with genome-wide examination of interpretation by ribosome profiling, we give a worldwide picture of SD-subordinate interpretation in plastids. We watched an articulated connection between's debilitated anticipated SD-aSD associations and diminished interpretation effectiveness. In any case, different lines of proof recommend that the quality of the SD-aSD cooperation isn't the main determinant of the translational yield of numerous plastid mRNAs. At long last, the interpretation productivity of mRNAs with solid optional structures around the begin codon is more reliant on the SD-aSD cooperation than feebly organized mRNAs. In this manner, our information uncovers the significance of the aSD in plastid interpretation inception, reveal chloroplast qualities whose interpretation is affected by SD-aSD associations, and give bits of knowledge into determinants of interpretation effectiveness in plastids.

Answer details

Subject: Biology

Level:High school

Key words

• Shine–Dalgarno sequence

• Role of Shine-Dalgarno sequence in translation

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