The Apgar Scale is a method used to assess the health of newborns. A score of three would indicate what?

Biology

1 answer:

Andrej [43]3 years ago

7 0

An Apgar score of 3 means the newborn requires immediate resuscitation.

Explanation:

The Apgar score is an assessing method done by the healthcare providers immediately the baby is born mostly within one to five minutes.

Apgar stands for the five basic parameters checked during this assessment:

A: Activity level or muscle tone
P: Pulse or heart rate
G: Grimace or response to stimulit or reflex irritability
A: Appearance or skin color
R: Respiratory or breathing rate

These parameters are scored on three points 0, 1, and 2

Apgar score indications:

From 0 to 3 indicates critically low condition

From 4 to 6 indicates a below normal condition

From 7 to 10 indicates normal condition.

The critically low condition usually occurs in premature, low birth-weight neonates and requires immediate intensive and resuscitative care measures to improve the newborn’s condition. This is highly necessary to improve the survival rate and prevent mortality.

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Like all enzymes, restriction endonucleases (REs) need to be handled very carefully to ensure that they do not denature. Which o

andriy [413]

Answer:

The correct answer is: d. REs activity is best preserved by freezing the RE and thawing it out for brief periods of time as needed.

Explanation:

Restriction enzymes can be defined as endonucleases that can recognize specific nucleotide sequence in a DNA (Deoxyribonucleic acid) sequence and cause breakage of phosphodiester bond in the DNA at the target site. Hence, it has the capacity of cleaving a DNA sequence.
These are made up of proteins which are required to be present in the accurately folded conformation in order to function.
Any form of alteration in the three-dimensional structure of the protein induced due to physical, chemical, mechanical or physiological factors can cause denaturation of the protein which in turn can render the protein non-functional.
Freezing of restriction enzymes (REs) directly without the use of glycerol will cause the movement of water molecules within the proteins as well layering of water molecules on the protein surface.
At low temperature these water molecules change to ice crystals which can alter the conformation of the protein or can cause breakage of peptide bonds in the proteins by physical force.
This can cause the protein to get denatured and hence non-functional.
Hence, it is never advisable to preserve REs by freezing directly without glycerol.
Glycerol forms a layer around the protein molecules and prevents the protein molecules from coming into direct contact with the water molecules and hence prevents protein denaturation.