Answer:
An iris will bloom during the summer months because the nights are shorter.
A poinsettia will bloom during early winter because the nights are longer.
Explanation:
I took it and its hormones
INDUSTRIALIST MATCH - UP: Match the correct man to their industry . 1. Carnegie A. Oil 2. Morgan B. Railroads 3. Rockefeller C. Electricity 4. Vanderbilt D. Steel 5. Westinghouse E. Finance
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1. Scientific method is the techniques that scientists used to carry out investigation process. It is made up of five steps, which are:
a. Observation: this is the stage in which a scientist make an observation to which he did not have an explanation.
b. Formation of a research question: in accordance to observation made, the scientist form a question on which his experiment will be based
c. Formation of hypothesis: at this stage the scientist offers a proposed explanation for the observation which he had made and state what he expects at the end of the experiment.
d. Conduction of the experiment: at this stage the scientist carry out the experiment using appropriate means.
e. Analysis of data and drawing of conclusion: this is last stage of the investigation, at this stage, the scientist analysis the results he obtained and draw a conclusion based on the results of the data.
2. Scientists used scientific method to carry out scientific investigations because the method minimize the influence of bias and prejudice in the experiments that they carry out. The method provides an objective, standardized approach to scientific investigation; this result in reproducible results which can be achieved by other scientists in every parts of the world. The method also helps to minimize errors during investigation.
Answer:
The correct answer is: d. REs activity is best preserved by freezing the RE and thawing it out for brief periods of time as needed.
Explanation:
- Restriction enzymes can be defined as endonucleases that can recognize specific nucleotide sequence in a DNA (Deoxyribonucleic acid) sequence and cause breakage of phosphodiester bond in the DNA at the target site. Hence, it has the capacity of cleaving a DNA sequence.
- These are made up of proteins which are required to be present in the accurately folded conformation in order to function.
- Any form of alteration in the three-dimensional structure of the protein induced due to physical, chemical, mechanical or physiological factors can cause denaturation of the protein which in turn can render the protein non-functional.
- Freezing of restriction enzymes (REs) directly without the use of glycerol will cause the movement of water molecules within the proteins as well layering of water molecules on the protein surface.
- At low temperature these water molecules change to ice crystals which can alter the conformation of the protein or can cause breakage of peptide bonds in the proteins by physical force.
- This can cause the protein to get denatured and hence non-functional.
- Hence, it is never advisable to preserve REs by freezing directly without glycerol.
- Glycerol forms a layer around the protein molecules and prevents the protein molecules from coming into direct contact with the water molecules and hence prevents protein denaturation.
