<span>Negatively charged R-groups in the primary structure.
This is because nickel acts as a cation, therefore you would be attracting anions, in this case it would be on the proteins that would be binding to the column. Once they bind to the matrix a was buffer of high salts can elute the proteins.</span>
Explanation:
Transpiration is the process of water movement through a plant and its evaporation from aerial parts, such as leaves, stems and flowers.it helps in photosynthesis.