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madam [21]
3 years ago
10

Use your understanding of direct and indirect ELISA to answer the following questions

Biology
1 answer:
a_sh-v [17]3 years ago
4 0

Answer:

Indirect ELISA will be the best approach to test the gonadotropin according to example.

Explanation:

Now, the scenario is that we are working on ribonuclease A (RNase A) which is an antigen and against this we produced mouse monoclonal antibodies to RNase A (anti-RNase A).

Note: No other laboratories have these monoclonal antibodies.

So, it means we don't have commercially available antibodies against RNase A and we are producing antibodies.

According to this scenario we will use indirect ELISA because firstly, we use antigen (RNase A) and then monoclonal antibodies against RNase A and after that we will use anti-monoclonal RNase A antibodies (secondary antibodies) with substrate to produce color according to concentration of antibodies.

Note: Secondary antibodies (anti-monoclonal RNase A antibodies) can be produced against RNase A antibodies.

We can't use sandwich ELISA because there is not any lab who has monoclonal antibodies against RNase A. So, first we use antigen then its antibodies and then against these antibodies we use secondary antibodies to determine the effectiveness of monoclonal antibodies against RNase A.

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Natural selection can cause microevolution (change in allele frequencies), with fitness-increasing alleles becoming more common in the population.

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We've already met a few different mechanisms of evolution. Genetic drift, migration, mutation...the list goes on. All of these mechanisms can make a population evolve, or change in its genetic makeup over generations.

But there's one mechanism of evolution that's a bit more famous than the others, and that's natural selection. What makes natural selection so special? Out of all the mechanisms of evolution, it's the only one that can consistently make populations adapted, or better-suited for their environment, over time.

You may have already seen natural selection as part of Darwin’s theory of evolution. In this article, we will dive deeper – in fact, deeper than Darwin himself could go. We will examine natural selection at the level of population genetics, in terms of allele, genotype, and phenotype frequencies.

Quick review of natural selection

Here is a quick reminder of how a population evolves by natural selection:

Organisms with heritable (genetically determined) features that help them survive and reproduce in a particular environment tend to leave more offspring than their peers.

If this continues over generations, the heritable features that aid survival and reproduction will become more and more common in the population.

The population will not only evolve (change in its genetic makeup and inherited traits), but will evolve in such a way that it becomes adapted, or better-suited, to its environment.

Explanation:

Here's my notes, i used and got it correct.

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