1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
madam [21]
3 years ago
10

Use your understanding of direct and indirect ELISA to answer the following questions

Biology
1 answer:
a_sh-v [17]3 years ago
4 0

Answer:

Indirect ELISA will be the best approach to test the gonadotropin according to example.

Explanation:

Now, the scenario is that we are working on ribonuclease A (RNase A) which is an antigen and against this we produced mouse monoclonal antibodies to RNase A (anti-RNase A).

Note: No other laboratories have these monoclonal antibodies.

So, it means we don't have commercially available antibodies against RNase A and we are producing antibodies.

According to this scenario we will use indirect ELISA because firstly, we use antigen (RNase A) and then monoclonal antibodies against RNase A and after that we will use anti-monoclonal RNase A antibodies (secondary antibodies) with substrate to produce color according to concentration of antibodies.

Note: Secondary antibodies (anti-monoclonal RNase A antibodies) can be produced against RNase A antibodies.

We can't use sandwich ELISA because there is not any lab who has monoclonal antibodies against RNase A. So, first we use antigen then its antibodies and then against these antibodies we use secondary antibodies to determine the effectiveness of monoclonal antibodies against RNase A.

You might be interested in
Which of the following statements correctly compares transcription in prokaryotes and eukaryotes?
aev [14]

Answer: Which of the following statements correctly compares transcription in prokaryotes and eukaryotes?

Explanation: It would be B

7 0
3 years ago
HELP!!!!!!!!!!!!!!!!!!!!!!!!!
madreJ [45]
4-Growth, maintenance, balance fluids, maintain proper hp

2-Actin, collagen
3 0
3 years ago
Read 2 more answers
The Old Faithful geyser found in Yellowstone Park is an example of _____. a feature resulting from earthquake activity a feature
Monica [59]

The given blank can be filled with a feature resulting from hot volcanic rock.  

The geysers refer to the rare characteristics on Earth, only about 1000 of them prevail, and more than half of those are situated in Yellowstone. For a geyser to develop, there must be a source of volcanic heat, enough groundwater, and a geologic plumbing system via which the heated water can escape.  

The absconding of water takes place when the groundwater is heated by the hot volcanic rocks. The intensifying steam bubbles push the water above via the fissures in the rock until they overflow from the geyser.  

With the absconding of the top layers of the water, the pressure on the hotter waters below diminishes, leading to a violent chain reaction of explosions, which expand the volume of the rising boiling water by up to 1500 times or more.  


4 0
2 years ago
(a)The same kind of plants grown & cultivated on a large scale at a place is called_____ (b) The first step before growing c
valkas [14]

Answer:

Following the answers are given below:-

Explanation:

(a) Plants of the same type that are grown and grown at a large-scale spot are referred to as crops. They are mostly cultivated for useful products.

(b) The very first step in the growth of plants is soil time to prepare to easy way to help water infiltration, appropriate oxygenation, and soil microbial economic expansion. Soil program includes the softening and attempting to turn of plant roots through all the plow procedure. The next step in growing crops is the collection of safe crops that is performed for some time by placing the seed in a water-filled flask.

(c) Infected seeds are lighter and hollow in strength and therefore rise at the top of the atmosphere. The good seeds calm down a bit at the edges, becoming heavier, which is used for sowing.

(d) Sunlight, water, and nutrients are essential requirements for seed germination and seedling growth. Seed seeding to the right depth and adequate spacing among seeds and seedlings confirm that these criteria are fulfilled.

(e) The principal Kharif crops in India are corn, maize, and cotton.

(f) individuals are used for producing plant manure.

(g) A combination of calcium acid phosphate and calcium sulfate formed by processing sulfuric phosphate stone: used primarily as a phosphate.

(h) Vegetables and lean meats are the two major categories of commercial grain crops. The word applies primarily to seeds from family Fabaceae plants, such as wheat, corn, and rice; seeds of non-edible vegetation types are often referred to as "grains."

4 0
3 years ago
How is the structure of the phospholipid so perfectly paired to the function of the cell membrane?
FrozenT [24]
The phospholipid molecule is both hydrophilic and hydrophobic which allows it to organize into a bilayer with the hydrophobic tales tucked away and the hydrophilic head exterior. This bilayer is also semipermeable allowing some small molecules in for the cell to use
6 0
2 years ago
Other questions:
  • Cellular differentiation progressively restricts cell fate because the unexpressed genes in the cell:
    6·1 answer
  • Suppose an experimenter becomes proficient with a technique that allows her to move DNA sequences within a prokaryotic genome.If
    8·1 answer
  • How can rock layers tell the geologic history of an area
    10·1 answer
  • The size of the earth is just right. If it were any bigger, its would be greater, which could result in an atmosphere different
    14·1 answer
  • Features that increase the likelihood of survival and reproduction by an organism in a particular environment are called
    15·1 answer
  • There are several properties of water that make Earth a suitable environment for life, including water's high boiling point and
    8·1 answer
  • In your own words, explain why a plant cell would need a larger vacuole than an animal cell.
    11·2 answers
  • Hey guys i am bòred wanna chàt​
    15·1 answer
  • Which of the following groups of organs all remove metabolic wastes from the human body?
    12·1 answer
  • in which case would facilitated diffusion be needed?A. With large polar molecules moving against the concentrationgradientB. Wit
    10·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!