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madam [21]
3 years ago
10

Use your understanding of direct and indirect ELISA to answer the following questions

Biology
1 answer:
a_sh-v [17]3 years ago
4 0

Answer:

Indirect ELISA will be the best approach to test the gonadotropin according to example.

Explanation:

Now, the scenario is that we are working on ribonuclease A (RNase A) which is an antigen and against this we produced mouse monoclonal antibodies to RNase A (anti-RNase A).

Note: No other laboratories have these monoclonal antibodies.

So, it means we don't have commercially available antibodies against RNase A and we are producing antibodies.

According to this scenario we will use indirect ELISA because firstly, we use antigen (RNase A) and then monoclonal antibodies against RNase A and after that we will use anti-monoclonal RNase A antibodies (secondary antibodies) with substrate to produce color according to concentration of antibodies.

Note: Secondary antibodies (anti-monoclonal RNase A antibodies) can be produced against RNase A antibodies.

We can't use sandwich ELISA because there is not any lab who has monoclonal antibodies against RNase A. So, first we use antigen then its antibodies and then against these antibodies we use secondary antibodies to determine the effectiveness of monoclonal antibodies against RNase A.

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The Q are 1. Emma is a 26-year-old professional woman who maintains her weight at 140 pounds by following a lacto-ovo vegetarian
bearhunter [10]

Answer:

Question 1

a) What is Emma’s recommended daily protein requirement? 50.4 grams

b. What percentage of Emma’s current total energy intake comes from protein?

13.33%

Does it fall within the recommended range in terms of the percentage of total energy intake?

Yes it does fall within the recommended range in terms of the percentage of total energy intake which is 10 - 35%

c. Emma is concerned about her intake of the essential fatty acid omega-3. Can you suggest for her two food items that she can include in her diet?

i. Walnuts

ii. Flaxseeds.

Question 2

2. Rank the following oils/fats according to the ease with which they would spoil, and explain how you arrived at your conclusion: coconut oil, flaxseed oil, olive oil.

According to the ease of spoilage:

Flaxseed oil – Coconut oil – Olive oil

Explanation:

a) What is Emma’s recommended daily protein requirement?

RDA of protein for adults is given as 0.8 g per kilogram of body weight of 0.36 per pounds of body weight.

Therefore, Emma's recommended daily protein intake is calculated as:

Her body weight in pounds × 0.36

Emma's body weight = 140 pounds

140 pounds × 0.36

= 50.4 grams.

Therefore, Emma’s recommended daily protein requirement is 50.4 grams of protein.

b. What percentage of Emma’s current total energy intake comes from protein?

Since 4 calories is the energy received from 1 gram of protein

In the question, we are told that Emma takes a daily intake of 40 grams of protein.

Therefore,

1 gram of protein = 4 calories

40 grams of protein =

40 × 4 = 160 calories.

The Lacto Ovo vegeterian diet, Emma is on gives her 1200 calories per day.

Hence, the percentage of Emma’s current total energy intake comes from protein is calculated as:

(Emma's daily energy in calories from protein ÷ Daily energy in calories supplied by her Lacto ovo vegetarian diet) × 100

= (160 calories ÷ 1200 calories) × 100

= 13.33%

c.

Omega -3- fatty acids are fatty acids that are very good for the body. Even though they are fatty acids, they do not clog the arteries thereby helping in preventing the occurrence of heart disease. They also lower the blood pressure in the body.

Foods that contain omega -3- fatty acids are fatty fish such as Salmon, Mackerel as well as nuts and seed like Walnuts, Flax seeds ( Flax seed oil).

Emma is concerned about her intake of the essential fatty acid omega-3. Can you suggest for her two food items that she can include in her diet?

Since Emma takes nuts, 2 food items she can include in her diet that are rich in Omega-3 fatty acids are:

i. Walnuts

ii. Flax seeds

2. Rank the following oils/fats according to the ease with which they would spoil, and explain how you arrived at your conclusion: coconut oil, flaxseed oil, olive oil.

According to the ease of spoilage:

Flaxseed oil – Coconut oil – Olive oil

Flaxseed oil spoils more easily than coconut oil and coconut oil spoils easily than Olive oil. This is based on their exposure to heat.

When heated up, Flaxseed oil breakdown more easily , followed by coconut oil.

Coconut oil has the tendency to become cloudy after been stored for a while.

This is why even though we can use but Flaxseed and Coconut oil for cooking , Olive oil is still the best because it has the tendency to withstand a large amount of heat and it is heat stable as well as more stable at even room temperature.

8 0
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