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madam [21]
3 years ago
10

Use your understanding of direct and indirect ELISA to answer the following questions

Biology
1 answer:
a_sh-v [17]3 years ago
4 0

Answer:

Indirect ELISA will be the best approach to test the gonadotropin according to example.

Explanation:

Now, the scenario is that we are working on ribonuclease A (RNase A) which is an antigen and against this we produced mouse monoclonal antibodies to RNase A (anti-RNase A).

Note: No other laboratories have these monoclonal antibodies.

So, it means we don't have commercially available antibodies against RNase A and we are producing antibodies.

According to this scenario we will use indirect ELISA because firstly, we use antigen (RNase A) and then monoclonal antibodies against RNase A and after that we will use anti-monoclonal RNase A antibodies (secondary antibodies) with substrate to produce color according to concentration of antibodies.

Note: Secondary antibodies (anti-monoclonal RNase A antibodies) can be produced against RNase A antibodies.

We can't use sandwich ELISA because there is not any lab who has monoclonal antibodies against RNase A. So, first we use antigen then its antibodies and then against these antibodies we use secondary antibodies to determine the effectiveness of monoclonal antibodies against RNase A.

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Sieve tubes are found in which vascular tissue
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4 years ago
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⦁ Está constituido mayoritariamente por agua; diferentes moléculas orgánicas, como carbohidratos; y moléculas inorgánicas, como
Marina86 [1]

The question is in another language so, English translation for the given question is as follows:

It is made up mostly of water; different organic molecules, such as carbohydrates; and inorganic molecules, such as mineral salts. It carries out most of the chemical reactions of the synthesis of molecules and obtaining energy. To which of the following cell structures does the above description correspond?

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Answer:

c) Cytoplasm

Explanation:

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The Cytoplasm is majorly made up of water, carbohydrates, salts, and proteins. Cytoplasm contains different molecules and enzymes that carries out most of the chemical reactions and obtain energy. Cytoplasm also helps in providing shape to the cell.

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6 0
3 years ago
Why do scientists use a control group in experimental design???
givi [52]
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Reason: If scientists did not have a controlled group then, they would have data for the test but they might know if the results changed or not. Scientists need a controlled group because the controlled group is like the original. They need the original group to help them know what improved or did not improve.

  For example, if you were testing whether or not different types of music helps with studying, then you will need a group that does not listen to music so you can know if different types of music actually improve or just make studying worse.

Having a controlled group helps you know the difference between observations in a easier way. The controlled group is not something people should ignore. It might seem useless at first but, when a person starts to finish up their project or test then, they will understand why a controlled group is important. By the time they do, it might be too late so make sure you people use a controlled group(Especially in the Science Projects).

(Hope this helps : ) )
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3 years ago
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