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madam [21]
3 years ago
10

Use your understanding of direct and indirect ELISA to answer the following questions

Biology
1 answer:
a_sh-v [17]3 years ago
4 0

Answer:

Indirect ELISA will be the best approach to test the gonadotropin according to example.

Explanation:

Now, the scenario is that we are working on ribonuclease A (RNase A) which is an antigen and against this we produced mouse monoclonal antibodies to RNase A (anti-RNase A).

Note: No other laboratories have these monoclonal antibodies.

So, it means we don't have commercially available antibodies against RNase A and we are producing antibodies.

According to this scenario we will use indirect ELISA because firstly, we use antigen (RNase A) and then monoclonal antibodies against RNase A and after that we will use anti-monoclonal RNase A antibodies (secondary antibodies) with substrate to produce color according to concentration of antibodies.

Note: Secondary antibodies (anti-monoclonal RNase A antibodies) can be produced against RNase A antibodies.

We can't use sandwich ELISA because there is not any lab who has monoclonal antibodies against RNase A. So, first we use antigen then its antibodies and then against these antibodies we use secondary antibodies to determine the effectiveness of monoclonal antibodies against RNase A.

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In plants, ______________ are defined as haploid (1n) cells that develop into gametophytes. Gametophytes, in turn, produce eithe
soldi70 [24.7K]

Answer: SPORES are defined as haploid (1n) cells which develops into gametophytes.

Gametophytes inturn produce either haploid (1n) male or females GAMETES that can fuse to form diploid ZYGOTES.

This basically explains what goes on in Meiosis.

8 0
3 years ago
Where are the richest deposits of oil and natural gas most likely located?
Maurinko [17]

Answer:

<em>The correct option is C) Continental shelves</em>

Explanation:

The continental shelf can be described as a part of a continent which is submerged in shallow water. This shallow water is known as a shelf. Hence, the name continental shelves.

Rich deposits of oil and natural gas can be present in this part of the sea. The continental shelves are of extreme commercial significance and are a commercial site for exploitation. The continental shelves can be rich in metallic and non- metallic ores and hydrocarbon products.

4 0
4 years ago
What are known causes of epigenetic effects, and how do they
OverLord2011 [107]

Answer:

Epigenetic effects can be caused by DNA heterochromatin and/or histone silence or activate.

As such, they can different chromosomes or certain chromosomal regions and be responsible for parental imprinting or influencing gene activity in oxidation and reduction.

Patterns of nucleotide demethylation and hypermethylation are often activate when cancer cells are compared to normal cells.

Explanation:

Cells acquire various patterns of gene expression during differentiation to adapt to a changing environment. Epigenetic and genetic alterations are considered as two independent mechanisms that participate in the onset and progression of cancer. Epigenetic mechanisms can be as important for biological events as genetic mechanisms, which do not imply a change in the DNA sequence, but do have an important role in the modification of gene expression.

4 0
3 years ago
When amino acids are linked together to form chains the molecule formed is called what?
choli [55]

Answer:

Within a protein, multiple amino acids are linked together by peptide bonds, thereby forming a long chain. Peptide bonds are formed by a biochemical reaction that extracts a water molecule as it joins the amino group of one amino acid to the carboxyl group of a neighboring amino acid.

Explanation:

7 0
3 years ago
Which of the following is NOT abiotic?
natali 33 [55]

Answer:

dead squirell

Explanation:

it used to be alive so that why

6 0
3 years ago
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