Choose a strategy to clone genes known only by a mutant phenotype in organisms with highly efficient transformation procedures a
nd relatively small genomes. a. cloning by complementation
b. direct sequencing of the mutant genome
c. PCR of the gene and transformation of an organism
d. cloning using a specific cloning vector
Option a (cloning by complementation) `would be the correct choice.
Explanation:
In species containing extremely efficient transforming techniques as well as comparatively small genomes, cloning by rearrangement testing is performed to clone gene products mostly by a mutated gene as the rearrangement test was being used to detect mutations.
This strategy for cloning through mechanism begins with identifying another yeast mutant that affects something like a plasmid representing a cloned genetic material.
The other options in question aren't relevant to the particular circumstance. Therefore the answer to the above seems to be the right one.
