Question

Choose a strategy to clone genes known only by a mutant phenotype in organisms with highly efficient transformation procedures and relatively small genomes.
a. cloning by complementation
b. direct sequencing of the mutant genome
c. PCR of the gene and transformation of an organism
d. cloning using a specific cloning vector

Answer 1

Answer:

Option a (cloning by complementation) `would be the correct choice.

Explanation:

• In species containing extremely efficient transforming techniques as well as comparatively small genomes, cloning by rearrangement testing is performed to clone gene products mostly by a mutated gene as the rearrangement test was being used to detect mutations.
• This strategy for cloning through mechanism begins with identifying another yeast mutant that affects something like a plasmid representing a cloned genetic material.

The other options in question aren't relevant to the particular circumstance. Therefore the answer to the above seems to be the right one.