Answer:
By way of introduction, A260/A280 ratio is use to measure Protein Contamination. This procedure was first described to measure protein purity in the presence of nucleic acids. However it is now commonly used to assess protein contamination of DNA. The method to determine the concentration of protein contamination by using A260/280 ratio method is well explained below by filling in the missing words.
Explanation:
The A260/A280 ratio method estimates protein __purity___ by measuring the absorbance maximum at 280nm caused by the amino acids__cytosine___, ____Adenine____, and ___Guanine_________ Since ___Spectrophotometer____ also absorbs in the UV range, we can correct for this contaminate by measuring the absorbance maximum at ____260nm______ and using the following equation: Concentration (µg/ml) = (A260 reading – A320 reading) × dilution factor × 50µg/ml___ When extrapolated from a standard curve, the Bradford data indicates the amount (in _ug__ ) of __unknown ___ protein found in a sample. If you know the volume of sample that was added to the assay then you can calculate the protein ____concentration ___ of the sample. The coomassie blue dye in the Bradford assay specifically binds primary ¬¬___sulfonic___ and __positive amines__ groups of the amino acid side groups of the proteins. The more the dye binds to the sample, the _anionic_ the blue color will be, and the absorbance at 595¬nm will be_shifted Amax___. A sample with an unusually __protein___ number of ___280nm of Tyrosine __ [give a specific example] amino acids will underestimate the total amount of protein present in the sample.
Answer:
Prophase II meiosis-chromosomes-6 and 12 DNA molecules per cell.
Explanation:
Meiosis is a unique type of division that occur in cells in which the chromosome number is decreased to half with the creation of haploid cells. It can be referred to as reduction division and it mostly occurs in organisms which go through sexual reproduction.
In prophase II , the nucleoli and nuclear membrane disappear.
The chromatids reduces in length to become short and thick .
The centromeres migrates to the polar regions and set the spindle fibres in place for the second meiotic division.
Therefore, at Prophase II of meiosis
The daughter cells are haploid. Its haploid cells should possess six chromosomes and 12 DNA molecules.
1. B, because Eukaryotes don't have cells walls or chloroplasts or large central vacuoles.
4. A, the vacuole gives the plant it's shape and stores wastes for the plant
Meiosis is a process where a single cell divides twice to produce four cells containing half the original amount of genetic information. That means that they will never be a copy of their parents because of their different genetic material. Characteristics, such as eye color, hair type and color, the shapes of facial features, and height and build, and attribute the combinations of these to the portions of DNA inherited from each parent which makes them different but still carry the DNA of their parents.
