Question

Salivary amylase is a protein secreted by the epithelial cells of the salivary glands to aid in carbohydrate digestion. Order the steps below for the generation of amylase in their correct order. The amylase polypeptide folds into a protein inside the ER lumen and is packaged in a vesicle destined for the Golgi
A release factor binds a stop codon on the amylase mRNA



Vesicles containing amylase leave the Golgi and fuse with plasma membrane to secrete protein from the cell



The growing polypeptide chain continues to insert itself inside the lumen of the ER



Ribosome is fully assembled and translation elongation proceeds



The tRNA charged with Met amino acid recognizes the AUG on the amylase mRNA bound to small ribosomal subunit



RNA polymerase binds to the amylase gene promoter



A mature mRNA is exported out of the nucleus



RNA elongation proceeds until RNA polymerase falls off the DNA



A poly A tail is added to the 3' of pre-mRNA

Answer 1

→ Answer: RNA polymerase binds to the amylase gene promoter

→RNA elongation proceeds until RNA polymerase falls off the DNA  

→A poly A tail is added to the 3' of pre-mRNA

 

→A mature mRNA is exported out of the nucleus

→ The tRNA charged with Met amino acid recognizes the AUG on the amylase mRNA bound to small ribosomal subunit

→Ribosome is fully assembled and translation elongation proceeds

 

→A release factor binds a stop codon on the amylase mRNA

→The growing polypeptide chain continues to insert itself inside the lumen of the ER

 

→Vesicles containing amylase leave the Golgi and fuse with plasma membrane to secrete protein from the cell