Answer: The sun generates energy from a process called nuclear fusion.
Explanation: I hopes this helps you out.
Symbiosis is the relationship of 2 organisms. The 3 major types are commensalism, mutalism, and parasitism. Hope that this helps!
Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
These genes could have arisen by gene duplication.
<h3>What is gene duplication?</h3>
Gene duplication is the process of copying a section of DNA that codes for a gene. A retro transposition event or a recombination mistake are both potential causes of gene duplication. As a result, the duplicate gene coding may experience a significant number of modifications throughout time. This might prevent the gene from functioning or, in other situations, give the creature a benefit.
Gene duplication is a phenomenon that can happen through a variety of ways:
- Ectopic Recombination: Uneven crossing-over between misaligned homologous chromosomes during meiosis might result in duplications. A duplication at the exchange site and a reciprocal deletion are the results of this recombination.
- Replication Slippage: Short genomic sequences can be duplicated as a result of the replication error known as replication slippage. DNA polymerase starts copying the DNA during replication, but eventually the polymerase separates from the DNA, causing replication to stall. The replicating strand is incorrectly aligned when the polymerase reattaches to the DNA strand, which accidentally results in several copies of the same portion.
- Aneuploidy: When a single chromosome's nondisjunction causes an abnormally high number of chromosomes, this condition is known as aneuploidy. Aneuploidy is frequently damaging and frequently causes spontaneous miscarriages in mammals. Some aneuploid people can survive. For instance, human trisomy 21 causes Down syndrome but is not lethal.
Learn more about gene duplication here:
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Growth and repair
creation of gametes (sex cells)
method of reproduction in unicellular organisms
Binary Fission - type of reproduction that occurs in bacterial cells, single celled organism splits and becomes two identical organisms
Chromosomes and DNA
Chromosomes are DNA wrapped around proteins to form an X-shaped structure.
The diagram will help you see the relationship.
1. Chromosomes are found in the nucleus
2. Chromosomes are made of DNA
3. Sections of chromosomes are called genes
DNA - deoxyribonucleic acid (it is the genetic code that contains all the information needed to build and maintain an organism)
Chromosome Structure
Chromosome Numbers
Each organism has a distinct number of chromosomes, in humans, every cell contains 46 chromosomes. Other organisms have different numbers, for instance, a dog has 78 chromosomes per cell.
Somatic Cells - body cells, such as muscle, skin, blood ...etc. These cells contain a complete set of chromosomes (46 in humans) and are called DIPLOID.
Sex Cells - also known as gametes. These cells contain half the number of chromosomes as body cells and are called HAPLOID
Chromosomes come in pairs, called Homologous Pairs (or homologs). Imagine homologs as a matching set, but they are not exacly alike, like a pair of shoes.
Diploid cells have 23 homologous pairs = total of 46
Haploid cells have 23 chromosomes (that are not paired) = total of 23
Homologous Chromosomes
Sex Determination
Chromosomes determine the sex of an offspring. In humans, a pair of chromosomes called SEX CHROMOSOMES determine the sex.
If you have XX sex chromosomes - you are female
If you have XY sex chromosomes - you are male
During fertilization, sperm cells will either contain an X or a Y chromosome (in addition to 22 other chromosomes - total of 23). If a sperm containing an X chromosome fertilizes an egg, the offspring will be female. If a sperm cell containing a Y chromosome fertilizes an egg, the offspring will be male.
Creation of a Zygote
When two sex cells, or gametes come together, the resulting fertilized egg is called a ZYGOTE
Zygotes are diploid and have the total 46 chromosomes (in humans)
Karyotype
A karyotype is a picture of a person's (or fetus) chromosomes. A karyotype is often done to determine if the offspring has the correct number of chromosomes. An incorrect number of chromosomes indicates that the child will have a condition, like Down Syndrome
Compare the Karyotypes below
Notice that a person with Down Syndrome has an extra chromosome #21. Instead of a pair, this person has 3 chromosomes - a condition called TRISOMY (tri = three)
Trisomy results when chromosomes fail to separate - NONDISJUNCTION - when sex cells are created. The resulting egg or sperm has 24 instead of the normal 23.
Other conditions result from having the wrong number of chromosomes:
Klinefelters Syndrome - XXY (sex chromosomes)
Edward Syndrome - Trisomy of chromosome #13