The appropriate sequence of steps involved in recombinant DNA technology are as under :-
1) Selected gene is isolated.
2) Restriction enzymes cut DNA into fragments.
3) Fragments of DNA are inserted into a vector.
4) Vector and recombinant DNA multiply.
5) Recombinant DNA is inserted into host.
In the very first step, the desired gene/genetic material for cloning is isolated from the cell. To do this the cell is lysed. Cells can be lysed by sonication or using chemicals like detergents.
In the second step, isolated DNA/gene is cut with the help pf biological scalpels known as restriction enzymes. The vector (usually it is a plasmid) is also cut down using restriction enzymes.
In the third step, the fragment of the restriction digested DNA (gene) and the vector are ligated together.
In the fourth step, this vector in which desired gene has been incorporated is cloned to generate various copies.
In the last step, this recombinant DNA is inserted into a host cell where is expressed i.e. in host cell this desired gene produces its product (protein).
Spontaneous neoplasms of the central nervous system of the cat, The statement is correct.
<h3>what are the major parts of the central nervous system ?</h3>
The central nervous system have two parts such as the brain and spinal cord, where the brain plays a crucial role in controlling most of the body functions like awareness, movements, thoughts, speech, and memory.
Spinal cord pathway involve in some reflex activities without the the involvement of brain, it is connected to brain stem a section of the brain where cranial nerve is present.
Nerve roots present in the spinal cord, this cord carries signals back and forth between the brain and the peripheral nerves, there is a fluid called Cerebrospinal fluid present between the brain and the spinal cord.
The cerebrospinal fluid flow between 2 meningeal layers called the pia matter and the arachnoid, another outermost thicker layer act as a protective shield called as dura matter.
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I think it's c: Quantity A is weight & Quantity B is mass
My guess would be "B" cell wall
<span>The main function of temporal lobes would be hearing. But temporal lobes do have multiple functions. These are present just above the ears on each side of our head. These lobes mainly control the hearing functions but they are as well related with short term memory, taste and smell functions of our body.</span>