If one wishes to clone a gene using typical restriction endonucleases, how does the restriction endonuclease identify the appropriate cut sites in the genome using the palindromic sequence.
Restriction endonucleases are a family of enzymes usually found in bacteria that cuts DNA at a specific sequence called recognition site. There are three type of restriction endonuclease Type I, Type II, and Type III.
Each endonuclease recognizes a particular sequence called palindromic sequence which is a sequence that reads the same sequence in both the strands either reading from the 3 prime end or 5 prime end.
The endonuclease cuts the palindromic sequence in such a way that it produces sticky ends.
To learn more about restriction endonucleases here
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<span> 1. </span>Selling 2. exploiting
According to the Endangered Species
Act, selling an endangered plant is prohibited. Likewise, exploiting the
habitat of an endangered animal is also a punishable act. The Endangered
Species Act is a law created for the conservation of threatened and endangered
plants and animals and the habitats in which they are found. The law prohibits any
act of taking and selling of any endangered Species.
Answer:
Subtract 4x4x from both sides of the equation.
y=16−4xy=16-4x
2x+3y=−22x+3y=-2
Replace all occurrences of yy in 2x+3y=−22x+3y=-2 with 16−4x16-4x.
y=16−4xy=16-4x
2x+3(16−4x)=−22x+3(16-4x)=-2
Simplify 2x+3(16−4x)2x+3(16-4x).
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y=16−4xy=16-4x
−10x+48=−2-10x+48=-2
Solve for xx in the second equation.
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y=16−4xy=16-4x
x=5x=5
Replace all occurrences of xx in y=16−4xy=16-4x with 55.
y=16−4⋅5y=16-4⋅5
x=5x=5
Simplify 16−4⋅516-4⋅5.
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y=−4y=-4
x=5x=5
The solution to the system of equations can be represented as a point.
(5,−4)(5,-4)
The result can be shown in multiple forms.
Point Form:
(5,−4)(5,-4)
Equation Form:
x=5,y=−4x=5,y=-4

4x+y=16,2x+3y=−24x+y=16,2x+3y=-2
Answer:
The electron transport chain (ETC) is located in the mitochodrial cristae. As electrons move from one protein complex to the other via redox reaction, energy is used to pump hydrogen ions (H+) from the matrix to the intermembrane space of the mitochondria. As hydrogen ions flow down the concentration gradient from the intermembrane space to the mitochodrial matrix, ATP is synthesized by the enzyme ATP synthase.
The ATP synthase complex can be compared to the gates of a dam. when the gates of a hydroelectric dam are opened, water rushes through, and electricity (energy) is produced. Similarly, when H+ ions flow down a gradient from intermembrane space into the matrix, the enzyme ATP synthase synthesizes ATP from ADP and inorganic phosphate or P<em>i. </em>This process is called chemiosmosis because ATP production is linked with the establishment of a proton (H+) gradient across the membranes.