When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone) and insert a linear fragment of DNA (insert) that has been cut by compatible restriction enzymes. An enzyme, DNA ligase, then covalently binds the plasmid to the new fragment thereby generating a complete, circular plasmid that can be easily maintained in a variety of biological systems. Read on for an in-depth breakdown of how to do perform restriction digests.
NOUN
the natural coloring matter of animal or plant tissue.
VERB
(pigmented)
color (something) with or as if with pigment.
"pigmented areas such as freckles"
The production of these crops is centered in the eastern third of the state but there are notable concentrations elsewhere, particularly in the river valleys of the Arkansas River (central Arkansas) and the Red River (southwest Arkansas).
<span>The genotype is
required for you to use a punnet square. In the construction of a punnet's
square, the genotypes of both parents are required. In the three by three
square, the first column shows the genetic representation of one parent and the
first row shows the genotype of the other parent. The two are combined, and
they yield a genetic combination of both parents, giving all the possible
outcomes of the offspring's genotypes. Using the punnet's square, you can be
able to identify the percentage of the offspring that will be heterozygous or
homozygous.</span>
Answer:
In biology, a spore is a unit of sexual or asexual reproduction that may be adapted for dispersal and for survival, often for extended periods of time, in unfavourable conditions. Spores form part of the life cycles of many plants, algae, fungi and protozoa.