Answer:
The correct order is dehydration, embed in wax, cut into sections, staining
Explanation:
There are certain proceedings needed to obtain stained sections of vegetable or animal tissues for their microscope observations.
These steps are:
- Obtention of the material: The tissue is cut to an adequate size.
- Fixation: When tissues are extracted from the organism, they suffer autolysis and putrefaction, so they need to be fixated in order to keep their cells in the best state possible. Fixation involves preserving the original morphological and molecular characteristics of the tissue. Fixation avoids autolysis, putrefaction, distortion, and retraction of cells and the tissue that could affect its volume or morphology.
- Dehydration. Once fixated, the fixator must be eliminated and the tissue is dehydrated by using a gradual series of solutions with alcohol in ascendant concentrations. Dehydration must be gradual to avoid tissue deformation.
- Inclusion. To obtain thin cuts that can be observed under the optic microscope, the tissues must be included in a consistent, firm substance, that might be either hydrophilic or hydrophobic. A hydrophobic medium is paraffin wax, that provides hardness and plasticity.
- Cut. The tissue included in wax must be cut in slides or sections thin enough to allow the diffusion and penetration of light. A microtome is used to perform these cuts. When using paraffine for tissue inclusion, the cuts are about 5 to 20 micrometers of thickness.
- Stain. Once the cuts are performed, paraffin wax must be eliminated. This can be done by using an organic solvent. Then the tissue must be stained. Hematoxylin and Eosin are the most common dyes. Animal tissues in general do not have any natural color, so they need to be stained to be observed.
April is the <u>fourth month</u> of the year in the Gregorian calendar, the fifth in the early Ju.lian, the first of four months to have a length of 30 days, and the second of five months to have a length of less than 31 days.
Answer:
Metabolizma, organizmalardaki yaşamı sürdüren kimyasal reaksiyonlar kümesidir. Üç ana ... Bu ortak kimya, hücrelerin küçük bir metabolik set kullanmasına izin verir ... Organik bileşikler (proteinler, lipitler ve karbonhidratlar) karbonun çoğunu içerir ... İlk olarak, bir enzimin bir yoldaki düzenlenmesi etkinliği nasıl
BU YARDIMCI OLUR UMARIM !
Answer:
Surface temperature and lower air pressure.
Explanation:
Surface temperature and lower air pressure are the types of data would a meteorologist need to collect in order to demonstrate the presence of a cyclone in a specific region. For the formation of cyclone, warm and moist air is required so surface temperature and lower air pressure are the data that gives information that the cyclone is present in a particular area or not.
What the hell is this a answer
