The answer to this question is 7 growth rate minus in the whole years
Chinese hamster ovary cell production of recombinant tissue‐type plasminogen activator (t‐PA) was increased by amplification of cotransfected dihydrofolate reeducates cDNA using stepwise adaptation to increasing methotrexate (MTX) concentrations. The highest producing clones were isolated at 5 μM MTX and yielded 26,000 U/106 cells/day t‐PA (43 μg/106 cells/day). Above 25 μM MTX, cell specific t‐PA production rates became increasingly variable and the cDNA copynumbers decreased. No apparent correlation between the cell specific t‐PA production rate and the growth rate was observed upon sub cloning of the amplified cells. When MTX selection was removed, the t‐PA production rate decreased up to tenfold within 40 days; this was accompanied by an up to 60% drop in cDNA copynumber. Subclones isolated after 108 days of culture in the absence of MTX were, on average, sixfold more stable than their parental cells. In culture without MTX, the maximum stable t‐PA production rate obtained (over 250 days) was 7000 ± 750 U/106cells/day (∼12 μg/106 cells/day), approximately threefold lower than the maximum unstable levels of production reached under selective pressure. Taken together, these results define a wide range of the highest t‐PA expression rates obtained under MTX selection, for which stable expression without selection has not been reported
The best material to use is foam, since it is the best insulator. An insulator will help slow the
transfer of heat from outside of the lunchbox to inside of the lunchbox. The extra layer will be of foam.
Explanation:
If we compare the specific heat capacity of rubber and foam it can be found that foam has high specific heat capacity, which indicates that it is the good insulator.
An insulator is a substance in which heat exchange between medium and surrounding is very low.
Jayla and her grandma wanted their food to be cold all morning so foam as an insulator is the best choice.
Thus, high specific heat energy means large amount of energy required to raise the temperature by 1 degree in response to the mass of the substance as per unit mass.
Instead of roots, threadlike structures called rhizoids.
have a nice day:)