Question

Current use Next Generation sequencing platforms (check all that apply): a. use sequencing by synthesis protocols b. use chemical sequencing protocols c. use sequencing by ligation protocols d. visualize the sequencing products through silver staining of gels

Answer 1

Answer:

The answer is - C. Use sequencing by ligation protocols

Explanation:

Next Generation Sequencing (NGS) is used to sequence entire genomes such that millions to billions of DNA strands can be sequenced in parallel and therefore reducing the need for fragment cloning methods. Unlike the Sanger sequencing the NGS is a high-throughput and it yields more throughput.

The current use Next Generation Sequencing Platforms use sequencing by ligation protocols which is a DNA sequencing method that uses the mismatch sensitivity of DNA ligase to discover the fundamental sequence of nucleotides in the DNA sequence and it involves the ligation of the probe to a universal primer.