The answer is - C. Use sequencing by ligation protocols
Explanation:
Next Generation Sequencing (NGS) is used to sequence entire genomes such that millions to billions of DNA strands can be sequenced in parallel and therefore reducing the need for fragment cloning methods. Unlike the Sanger sequencing the NGS is a high-throughput and it yields more throughput.
The current use Next Generation Sequencing Platforms use sequencing by ligation protocols which is a DNA sequencing method that uses the mismatch sensitivity of DNA ligase to discover the fundamental sequence of nucleotides in the DNA sequence and it involves the ligation of the probe to a universal primer.
It starts as a raindrop which is rain than it goes to the ocean,rivers,puddles or any body of water and from there it evaporates which makes the clouds and than it starts all over when the clouds already got enough water.
