1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
Anna71 [15]
3 years ago
14

Please give a small paragraph quickly summarizing the what, when and how of each of the following techniques: PCR, DNA gel elect

rophoresis, Recombinant DNA, Cloning, Northern blots, Southern blots, Western blots, Antibody production (polyclonal and monoclonal), ELISA, FRET, FRAP, FACS, Fractionation by centrifugation, Chromatography, Fluorescence microscopy, Coomassie staining, Silver staining, His tag, GST tag, Confocal microscopy, FISH, PCR, DNA library, cDNA library, Microarrays, Sanger sequencing, GFP, Bright field microscopy, DIC microscopy, Phase contrast microscopy, Fluorescence microscopy, Transmission electron microscopy, Scanning electron microscopy, X ray crystallography, 2D PAGE electrophoresis, NMR, Mass spectrometry.
Biology
1 answer:
Anuta_ua [19.1K]3 years ago
4 0

Answer:

All are used to resolve questions in molecular and biochemistry/biotechnology

Explanation:

PCR: resolution of an amplicong on agarose gel to chech size after thermocycling

DNA gel electrophoresis,

Recombinant DNA, A DNA fragment that it attached to another such as a reporter, commonly used is GFP attached to protein of interest to track movement

Cloning, Duplicate an amplicon, insert into a vector, transform this vector into a bacteria that is designed to make multiple copies of itself

Northern blots, Method used to detect different sizes RNA from a mixture of other products

Southern blots, method used to detect different sizes of DNA similar to the idea of a Northern Blot

Western blots, Resolution of protein sizes by running the protein through an acrylamide gel in an ionic buffer

Antibody production (polyclonal and monoclonal),  Generating an antigen against a protein using different parts of the same protein (polyclonal) or only a specific sequence of the protein not a variety of antigens from the full length (monoclonal)

ELISA, enzyme-linked immunosorbent assay is used to check the presence of a protein

FRET, Transmission energy of one molecule to another, it is usually included in the detection of colors in fluorescence microscopy

FRAP, this method will is called Fluorescence recovery after photobleaching, a microscospy measurement

FACS, this is a type of cell sorting Fluorescence-activated cell sorting

Fractionation by centrifugation, Lysis of agents such as cells that can be lysed by spinning in a centrifuge

Chromatography, separation of chemical thru a media by colors

Fluorescence microscopy,  use of fluorophore to detect specimen under a microscope a specific wavelength

Coomassie staining,  procedure used to stain an acrylamide gel or membrane to show protein presence

Silver staining, use of a silver colloid to change the way proteins are seen on a Western blot or under a microscope

His tag, a string of histidine residues attached to a protein for easy manipulation/detection

GST tag, Glutathione S-transferases is a group of proteins used in protein purification an option other than His tag

Confocal microscopy,

FISH, The generation of a nucleotide probe used in DNA sequence detection in histology

PCR, Polymerized chain reaction used to amplify selected region of DNA

DNA library, the collection of gDNA of a specific specie or tissue

cDNA library, collection of the coding sequence of a organism/tissue

Microarrays, the platform used to detect thousands of gene sequences at once

Sanger sequencing, Method used to derrive DNA sequence developed by Frederick Sanger by incorporating a single nucleotide at a time

GFP, Green fluorescent protein, a reporter protein used in recombination protein creation

Bright field microscopy, microscopy that uses light or natural light to detect samples

DIC microscopy, Differential interference contrast is used to detect and enhance the different levels of contrast of a specimen

Phase contrast microscopy, Microscopy technique used to detect the different states of matter of a specimen

Fluorescence microscopy, use of fluorophore attached to sample for investigation

Transmission electron microscopy, uses beams of electrons to pass through a sample to then create an image

Scanning electron microscopy,  the surface of a sample is scanned with a beam of electrons to generate an image

X ray crystallography, determining a structure of a protein my using an x-ray technique

2D PAGE electrophoresis,  separation of proteins by two phases sizes and charges

NMR,  Nuclear magnetic resonance, spinning of all the nuclei and measurement of the energy that it gives off.

Mass spectrometry Protein sequencing technique based on weight

You might be interested in
Which stage of the cell cycle involves the division of the cytoplasm?
Aloiza [94]

Answer:

Cytokinesis

Explanation:

5 0
3 years ago
Read 2 more answers
Concerns over a global dependence on fossil fuels include environmental, economic, and social consequences.
Natasha2012 [34]

True

Explanation:

Concerns over a global dependence on fossil fuels include environmental, economic and social consequences. The effect of reliance on fossil fuels cuts across on almost all aspect of life.

  • Environmental concerns include pollution, acid rain, global warming e.t.c
  • Economic consequences involves monopoly of the energy market, scarcity, inflation, corruption e.t.c
  • Social consequences include reduced standard of living, poverty, e.t.c

Fossil fuel is a non-renewable source of energy.

Learn more:

non-renewable energy brainly.com/question/6944540

#learnwithBrainly

5 0
3 years ago
Read 2 more answers
Will a wound will take a longer time to heal if an individual is lack of carbohydrate?
mezya [45]

Answer:

Carbohydrates Role in the body:

Glucose is necessary in wound healing for a number of reasons. These include: Providing energy for leucocytes and macrophages (white blood cells). Stimulates fibroblast growth etc

Wound healing implicates several biological and molecular events, such as coagulation, inflammation, migration-proliferation, and remodeling. Here, we provide an overview of the effects of malnutrition and specific nutrients on this process, focusing on the beneficial effects of curcumin. We have summarized that protein loss may negatively affect the whole immune process, while adequate intake of carbohydrates is necessary for fibroblast migration during the proliferative phase. 

Explanation:

Hope this helps, Brainliness please and thank you

7 0
2 years ago
What substance must plants produce in order to make lipids?
Mariana [72]
Amino acids are produces
8 0
3 years ago
What is osmosis active transport
wel

Answer:

Active transport is the movement of particles from an area of low concentration to an area of high concentration using a force.

7 0
3 years ago
Other questions:
  • A weight-training program that gradually increases the resistance lifted relies on the ________ principle to build strength
    5·1 answer
  • How hard the blood pushes on the inside of the blood vessels is called what?
    8·2 answers
  • Which of the following systems or structures is correctly paired with its function?
    9·1 answer
  • Why is it necessary to group herbs into garden beds?
    9·1 answer
  • Hydrolysis of GTP to GDP by a GTPase can cause a change in which aspect(s) of that protein's structure?
    5·1 answer
  • The table shows the specific heat of four substances—brick, dry soil, paper, and water. If all four substances were exposed to s
    11·2 answers
  • How do you think scientists use satellites to collect weather data
    10·1 answer
  • 1. Which statement about the current U.S. Supreme
    14·1 answer
  • Explain how DNA is used to make a protein.
    13·1 answer
  • I need help please can anyone help?
    5·2 answers
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!