1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
Anna71 [15]
3 years ago
14

Please give a small paragraph quickly summarizing the what, when and how of each of the following techniques: PCR, DNA gel elect

rophoresis, Recombinant DNA, Cloning, Northern blots, Southern blots, Western blots, Antibody production (polyclonal and monoclonal), ELISA, FRET, FRAP, FACS, Fractionation by centrifugation, Chromatography, Fluorescence microscopy, Coomassie staining, Silver staining, His tag, GST tag, Confocal microscopy, FISH, PCR, DNA library, cDNA library, Microarrays, Sanger sequencing, GFP, Bright field microscopy, DIC microscopy, Phase contrast microscopy, Fluorescence microscopy, Transmission electron microscopy, Scanning electron microscopy, X ray crystallography, 2D PAGE electrophoresis, NMR, Mass spectrometry.
Biology
1 answer:
Anuta_ua [19.1K]3 years ago
4 0

Answer:

All are used to resolve questions in molecular and biochemistry/biotechnology

Explanation:

PCR: resolution of an amplicong on agarose gel to chech size after thermocycling

DNA gel electrophoresis,

Recombinant DNA, A DNA fragment that it attached to another such as a reporter, commonly used is GFP attached to protein of interest to track movement

Cloning, Duplicate an amplicon, insert into a vector, transform this vector into a bacteria that is designed to make multiple copies of itself

Northern blots, Method used to detect different sizes RNA from a mixture of other products

Southern blots, method used to detect different sizes of DNA similar to the idea of a Northern Blot

Western blots, Resolution of protein sizes by running the protein through an acrylamide gel in an ionic buffer

Antibody production (polyclonal and monoclonal),  Generating an antigen against a protein using different parts of the same protein (polyclonal) or only a specific sequence of the protein not a variety of antigens from the full length (monoclonal)

ELISA, enzyme-linked immunosorbent assay is used to check the presence of a protein

FRET, Transmission energy of one molecule to another, it is usually included in the detection of colors in fluorescence microscopy

FRAP, this method will is called Fluorescence recovery after photobleaching, a microscospy measurement

FACS, this is a type of cell sorting Fluorescence-activated cell sorting

Fractionation by centrifugation, Lysis of agents such as cells that can be lysed by spinning in a centrifuge

Chromatography, separation of chemical thru a media by colors

Fluorescence microscopy,  use of fluorophore to detect specimen under a microscope a specific wavelength

Coomassie staining,  procedure used to stain an acrylamide gel or membrane to show protein presence

Silver staining, use of a silver colloid to change the way proteins are seen on a Western blot or under a microscope

His tag, a string of histidine residues attached to a protein for easy manipulation/detection

GST tag, Glutathione S-transferases is a group of proteins used in protein purification an option other than His tag

Confocal microscopy,

FISH, The generation of a nucleotide probe used in DNA sequence detection in histology

PCR, Polymerized chain reaction used to amplify selected region of DNA

DNA library, the collection of gDNA of a specific specie or tissue

cDNA library, collection of the coding sequence of a organism/tissue

Microarrays, the platform used to detect thousands of gene sequences at once

Sanger sequencing, Method used to derrive DNA sequence developed by Frederick Sanger by incorporating a single nucleotide at a time

GFP, Green fluorescent protein, a reporter protein used in recombination protein creation

Bright field microscopy, microscopy that uses light or natural light to detect samples

DIC microscopy, Differential interference contrast is used to detect and enhance the different levels of contrast of a specimen

Phase contrast microscopy, Microscopy technique used to detect the different states of matter of a specimen

Fluorescence microscopy, use of fluorophore attached to sample for investigation

Transmission electron microscopy, uses beams of electrons to pass through a sample to then create an image

Scanning electron microscopy,  the surface of a sample is scanned with a beam of electrons to generate an image

X ray crystallography, determining a structure of a protein my using an x-ray technique

2D PAGE electrophoresis,  separation of proteins by two phases sizes and charges

NMR,  Nuclear magnetic resonance, spinning of all the nuclei and measurement of the energy that it gives off.

Mass spectrometry Protein sequencing technique based on weight

You might be interested in
A dna molecule containing 32% thymine would contain how much cytosine?
anygoal [31]
18% because C pairs with G. A pairs with T. T and C are not complementary base pairs, but we do know that if a molecule consists of 32% T, it must have 32% A as well.

32 + 32 = 64

100 - 64 = 36

36 ÷ 2 = 18

There must be 18% of C.
8 0
3 years ago
A good conclusion restates the hypothesis so that the reader
SSSSS [86.1K]
A good conclusion restates the hypothesis so that the reader will get was was the hypothesis made by the person and weather it was correct or not, if not then it has logical explanation to it. Conclusion also tells/summary of your project/essay
7 0
3 years ago
If a virus got into your body, which systems might work together to fight it?
vredina [299]
Maybe immune system and respiratory system
6 0
3 years ago
The picture below shows a type of fish that is adapted to live in the weedy areas of freshwater lakes. image Which best describe
marissa [1.9K]
I think the answer would be A because the fish does look like a plant
4 0
3 years ago
Should women who are not pregnant and men be concerned about Zika infection
dalvyx [7]

Answer:

Women should stand by  at least two months after travel (or 2 months after  symptoms started to manifest if they get sick) before trying to get pregnant.The waiting period is longer for men because Zika stays in semen longer than in other body fluids.

Explanation:

3 0
3 years ago
Other questions:
  • Which is considered the foundation level of organization in an environment? community ecosystem organism population
    9·2 answers
  • WILL GIVE BRAINLIEST!!! URGENT!!! 25 pts!!!!! were any specific investigations conducted for proposing Clements successional the
    6·2 answers
  • The function of which human organ is most like the cell walls of bacteria?
    10·1 answer
  • Good guys finish last.
    11·1 answer
  • A lizard and bat have similar forlimbs which better adapt to theor different environment, but they are used in different ways th
    9·1 answer
  • Pill bugs" or "sow bugs", often found under rocks and logs in moist places, have the ability to roll up into a ball when disturb
    11·1 answer
  • Acciones saludables y no saludables
    15·2 answers
  • Pls answer these. I crossed out 13 and 15 because i already answered them. And i BEG YOU don’t just answer for points. I’ll give
    10·2 answers
  • PLEASE HELP ME WITH THESE THREE QUESTIONS ASAP!!!!!
    15·1 answer
  • What are some indicators that the overall climate of earth is getting warmer?.
    14·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!