Question 2 is false because it is always opposite.
Question 3 Ionic charge in which valence electrons are lost from one atom and gained by another one hoped it helped.
Each (both large and small) colony is carefully counted (using magnifying colony counter if needed). Each colony represents a “colony-forming unit” (CFU). For accurate counts, the optimum count should be within the range of 30-300 colonies/plate. To insure a countable plate a series of dilutions should be plated.