CO2 VALUE was 278 which means it was 16.8 and 9
Hi
Below are five major steps of DNA or gene cloning:
1: You chose the gene or specific piece of DNA you need to clone and cut the gene with restriction enzymes from the source organism.
2: You need to choose a vector for the process of cloning and you will cut the vector with same restriction enzymes through which you have cut your target DNA sequence to be inserted into the vector.
3: You place the target gene into the vector and join or seal the gene with vector by using an enzyme called DNA ligase.
4: You introduce the vector with your target gene into a suitable host organism such as yeast or bacteria through the process of Transformation. In this process host organism takes up the vector containing your target gene and starts replicating the target DNA along with their own DNA and thus creating millions of copies of target gene .
5: In the last step, the DNA or target gene is isolated from host organism and purified and is ready to be used since its quantity has been enormously increased through the process of cloning.
The cloning is also called as recombinant DNA technology and is the main process that is being used in the production of insulin for diabetes patients. You can see below image for better understanding.
Hope it help!
Answer:
Hence, during infection in vivo, a noncytopathic virus may turn off the "differentiation" or "luxury" function of a cell while not killing that cell (loss of vital function). This is turn can disrupt homeostasis and cause disease
hope it helps you
Answer:
Cells come from other living cells
All living things are composed of one or more cells
The cell is the basic unit of life.
Answer:
2% of the progeny will be double crossovers for the trihybrid test cross
Explanation:
By knowing the positions of genes, we can estimate the distances in MU between them per region.
- Genes A and B are 10 map units apart (Region I)
- Genes B and C are 20 map units apart (Region II)
- Genes A and C are 30 map units apart
----A-------10MU--------B-------------20MU-------------C---
Region I Region II
We can estimate the recombination frequencies by dividing each distance by 100.
• recombination frequency of A-B region = 10MU / 100 = 0.10
• recombination frequency of B-C region = 20MU / 100 = 0.20
Now that we know the recombination frequencies in each region, we can calculate the expected double recombinant frequency, EDRF, like this:
EDRF = recombination frequency in region I x recombination frequency in region II.
EDRF = 0.10 x 0.20 = 0.02
2% of the progeny will be double crossovers for the trihybrid test cross