Hi,
Recombinant DNA technology is the process in which a target gene of interest is replicated by inserting it into a vector and creating a recombinant DNA. This recombinant DNA is then returned into an expression system where it is replicated several times to give rise to multiple copies of our desired gene or that gene product.
One of the very important role for the execution of this technology is performed by Restriction endonuclease enzymes. These are the enzymes that have ability to cut the DNA fragment at specific sequence into fragments of different lengths called restriction fragments.
- They play a very important role in recombinant DNA technology:
- They can map the location of specific restriction sites where the target sequence is identified and cleaved.
- They cut down the DNA at specific sites called restriction sites. The host DNA in which foreign gene or target gene is inserted is also cut with the same restriction enzymes. So in the recombinant DNA technology, restriction enzymes act like a molecular scissor which identify and cut certain DNA sequences as per or requirement.
- The target sequence can be cut from multiple places by restriction enzymes since our target sequence can be present multiple times in the DNA of organism.
- When restriction enzymes cut the DNA, they leave sticky or blunt ends at that site in such a way that these ends are able to bind with the complementary bases of vector DNA when introduced.
- Then another enzyme DNA ligase seals the ends of target DNA and vector DNA making a whole recombinant DNA that is ready to be cloned and progress further steps of recombinant DNA technology.
<em>You can see attached image for better understanding.</em>
Hope it help!
Answer:
The right order is 3, 1, 2.
Explanation:
The most superficial layer is 3, which describes the Cornea. This layer, which is first hit by light, is made of transparent and resistant fabric. It protects the eyeball from trauma and contamination and helps shape the globe and refract light.
Then, the layer described as 1 is the pigmented part that has melanocytes, which may be the uvea that consists of the whole of the iris, choroid membrane and ciliary processes.
Finally, the deepest layer in this question describes the lens, which is a gelatinous biconvex lens. This important structure enables the eye to focus on objects.
There may not be a diagram, but the organelle, ribosome, helps mRNA and tRNA bind together. :)
Answer:
Ministry of climate change, Ministry of Commerce and Textile Industry, Ministry of Communications and Ministry of Defense
Explanation:
Ministry of climate change, Ministry of Commerce and Textile Industry, Ministry of Communications and Ministry of Defense are the four departments of national government. Every department has their own functions and responsibilities which is done by the officials of that departments. These departments has a great role in running of the government efficiently.
When you breathe in, or inhale, your diaphragm contracts and moves downward. This increases the space in your chest cavity, and your lungs expand into it. The muscles between your ribs also help enlarge the chest cavity. They contract to pull your rib cage both upward and outward when you inhale