Answer:
The correct option is 3. "At pH 6.5 the enyzme is 50% active"
Explanation:
For the titratable group to be protonated and cause the enzyme to be in the active state, it needs to have gained a hydrogen cation (H+). In order for that to happen, there must be enough hydrogen cations in the environment of the enzyme, and hence, an acidic pH is required in this case.
The process of making multiple copies of a gene by inserting it into a host genome and culturing the host is an example of gene cloning.
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