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Komok [63]
4 years ago
8

Carbon atoms have four electrons in their outer shell. this means that a single carbon atom can form up to bonds with other atom

s
Biology
2 answers:
Gre4nikov [31]4 years ago
5 0
This means that a single carbon atom can form up to tetravalent bonds with other atoms
Yakvenalex [24]4 years ago
5 0

4 !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!

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4 years ago
Why choose that gene for PCR in the bacterial identification lab?
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Answer:

Background

During the course of a bacterial infection, the rapid identification of the causative agent(s) is necessary for the determination of effective treatment options. We have developed a method based on a modified broad-range PCR and an oligonucleotide microarray for the simultaneous detection and identification of 12 bacterial pathogens at the species level. The broad-range PCR primer mixture was designed using conserved regions of the bacterial topoisomerase genes gyrB and parE. The primer design allowed the use of a novel DNA amplification method, which produced labeled, single-stranded DNA suitable for microarray hybridization. The probes on the microarray were designed from the alignments of species- or genus-specific variable regions of the gyrB and parE genes flanked by the primers. We included mecA-specific primers and probes in the same assay to indicate the presence of methicillin resistance in the bacterial species. The feasibility of this assay in routine diagnostic testing was evaluated using 146 blood culture positive and 40 blood culture negative samples.

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Results

Comparison of our results with those of a conventional culture-based method revealed a sensitivity of 96% (initial sensitivity of 82%) and specificity of 98%. Furthermore, only one cross-reaction was observed upon investigating 102 culture isolates from 70 untargeted bacteria. The total assay time was only three hours, including the time required for the DNA extraction, PCR and microarray steps in sequence.

3 0
3 years ago
Air at the equator is warm and has a ______density, which creates ________ in comparison to the air at the poles.
almond37 [142]

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Lower, low pressure

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The temperature at equator are mostly higher than at the poles almost all times of year. This high temperature causes air at the equator to warm up quickly and thus, the air density reduces and it becomes lighter. Now, light air rises upwards. Thus, creating a low pressure region at equators. This low pressure causes the air near the poles gush towards the equator.

Hence, the blanks will be filled with the words

Lower, low pressure.

5 0
3 years ago
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