1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
enyata [817]
3 years ago
10

Oct4 is an embryonic stem cell specific transcription factor. ESCs grown in petri dishes are fed media that contain basic fibrob

last growth factor (bFGF) which keeps the ESCs undifferentiated. They examine Oct4 mRNA levels in human ESCs (hESCs), hESCs with exogenous miR-223 (micro RNA 223), hESCs with exogenous miR-223 that do not have bFGF in the media, and hESCs without bFGF but with miR-223 inhibitor.Which technique would allow for measurement of Oct4 mRNA levels
Biology
1 answer:
VikaD [51]3 years ago
6 0

Answer:

You can use the quantitative Reverse Transcription Polymerase Chain Reaction PCR (RT-qPCR) method in order to analyze the Oct4 mRNA level.  

Explanation:  

RT-PCR is the gold standard technique to analyze the expression levels of one particular messenger RNA (mRNA) molecule. In this technique, the total RNA is firstly extracted and transcribed into complementary DNA (cDNA). Subsequently, the gene sequence of interest is then amplified in a standard PCR technique that uses as control one or more genes in order to measure the relative expression levels of the target mRNA, in this case, for example, the Oct4 mRNA.

You might be interested in
How are the levels of organization in animals and plants different?
coldgirl [10]
Because one of them dies and another one doesn’t
8 0
3 years ago
Be able to compare/contrast humoral immunity with cellular immunity.
aksik [14]

Solution:

The difference between them are:


The humoral system of immunity is also called the antibody-mediated system because of its use of specific immune-system structures called antibodies. The first stage in the humoral pathway of immunity is the ingestion (phagocytosis) of foreign matter by special blood cells called macrophages. The macrophages digest the infectious agent and then display some of its components on their surfaces. Cells called helper-T cells recognize this presentation, activate their immune response, and multiply rapidly. While,


The cell-mediated immune response involves cytotoxic T-cells, or killer-T cells. Body cells that have been infected by foreign matter often present components of that material on their surfaces. Killer-T cells recognize these displays and respond by ingesting or otherwise destroying the infected cell. Killer-T cells are also important in the body's defenses against parasites, fungi, protozoans, and other larger cells that might have found their way into the body. The killer-T cells recognize these large invaders by their foreign proteins and then destroy them.  

This is the required answer.

6 0
3 years ago
What is the difference between ionic bond and colvant bond
Darina [25.2K]
Ionic bond is a compound made up of a meta and a non-metal eg NaCl (sodium chloride)
covalent bond is made up of two non-metals eg H2O (water)
3 0
3 years ago
Explain the relationship between crossing over and genetic variation
7nadin3 [17]

Answer:

When homologous chromosomes form pairs during prophase I of meiosis I, crossing-over can occur. Crossing-over is the exchange of genetic material between homologous chromosomes. It results in new combinations of genes on each chromosome. It is obviously another source of genetic variation in offspring.

Hope this helps you. Do mark me as brainliest.

8 0
3 years ago
If the sequence of bases on one half of a DNA molecule is GATTACA, what is the sequence of bases on the opposite side (from left
AURORKA [14]
The sequence of bases on the other half of the DNA molecule would be CTAATGT from left to right, because each cytosine base pairs with a corresponding guanine base, and each adenine base pairs with a corresponding thymine base. 
4 0
3 years ago
Other questions:
  • Suppose that the coding region of a gene contains 1,800 base pairs, with 570 in exon 1, 420 in exon 2, and 810 in exon 3 (not co
    13·1 answer
  • Which of the following is true about skin bones muscles the heart intestines
    7·1 answer
  • What would you use to measure the size of a force?
    9·2 answers
  • In the models that describe population growth, r stands for _____.
    6·1 answer
  • What is the result of cell division?
    14·1 answer
  • Water dish soap milk witch has a higher density​
    11·2 answers
  • What property is shared by many gemstones, such as diamonds, rubies, sapphires, emeralds, and topaz?
    9·2 answers
  • Why is there more than one single locus probe used in actual paternity DNA test?
    15·1 answer
  • Which of the following is not associated with prions?
    5·1 answer
  • PLEASE HELP ASAP!!!
    8·2 answers
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!