1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
enyata [817]
3 years ago
10

Oct4 is an embryonic stem cell specific transcription factor. ESCs grown in petri dishes are fed media that contain basic fibrob

last growth factor (bFGF) which keeps the ESCs undifferentiated. They examine Oct4 mRNA levels in human ESCs (hESCs), hESCs with exogenous miR-223 (micro RNA 223), hESCs with exogenous miR-223 that do not have bFGF in the media, and hESCs without bFGF but with miR-223 inhibitor.Which technique would allow for measurement of Oct4 mRNA levels
Biology
1 answer:
VikaD [51]3 years ago
6 0

Answer:

You can use the quantitative Reverse Transcription Polymerase Chain Reaction PCR (RT-qPCR) method in order to analyze the Oct4 mRNA level.  

Explanation:  

RT-PCR is the gold standard technique to analyze the expression levels of one particular messenger RNA (mRNA) molecule. In this technique, the total RNA is firstly extracted and transcribed into complementary DNA (cDNA). Subsequently, the gene sequence of interest is then amplified in a standard PCR technique that uses as control one or more genes in order to measure the relative expression levels of the target mRNA, in this case, for example, the Oct4 mRNA.

You might be interested in
Suppose that Rose has myotonic dystrophy and shows traits such as uncontrolled muscle activity. Rose\'s mother, Becky, is dystro
Bogdan [553]

The six pedigree symbols with the corresponding names of the individuals in Rose's family will be like  2nd - Rose; 7th - Becky; 4th - Dave; 8th - Mike; 10th - Pat and 13th - Erik.

The second will be Rose because Becky's father, who is Rose's husband, is healthy and Jess, Becky's mother, has myotonic dystrophy. The seventh person is Becky, who has myotonic dystrophy and is married to Mike, who is healthy. Dave, who has myotonic dystrophy, is the fourth.

Mike, who is normal and married to Becky who has the condition or is impacted, comes in at number eight. Pat (unaffected), the daughter of Dave and Mary, is ranked tenth. Erik (affected), the son of Becky and Mike, is the 13th person on the list.

Myotonic dystrophy is an autosomal dominant condition that is passed vertically from parents to offspring. This illness affects both men and women equally and does not pass down through generations.

To learn more about Myotonic dystrophy visit: brainly.com/question/29483072

#SPJ4

4 0
1 year ago
Catabolism is a metabolic process in which protein, fats, and carbohydrates are broken down into simple molecules.truefalse
Mashutka [201]
The answer is true, since catabolism is the breakdown of more complex compounds into more simpler ones.
7 0
4 years ago
PLEASE HELP ME IM BEGGING YOU!!!!!!!!!!!!!!! Water plays an important role in the chemical weathering of rock. In 3–5 sentences,
Advocard [28]

Answer:

Water along with carbon dioxide and oxygen are the agents of chemical weathering to support that statement.

Explanation:

  • Chemical weathering is the continuous process that makes the rocks weak and decompose by changing its composition.
  • Water is the main agent for chemical weathering, the molecules of water separates the ions from their existing compounds and edges them.
  • Water has the ability to completely dissolve some minerals. for example, salt.
  • Oxygen also takes part in chemical weathering by the process called as oxidation.
  • Similarly carbon dioxide initiates weathering by the acid rain.
5 0
3 years ago
What is a catalyst in Chemical reaction?<br>​
tatiyna

Answer:

Catalyst, in chemistry, any substance that increases the rate of a reaction without itself being consumed. Enzymes are naturally occurring catalysts responsible for many essential biochemical reactions.

6 0
3 years ago
Read 2 more answers
How do scientist predict the patterns of heredity?
Nookie1986 [14]

Answer:

The classic tool to predict the patterns of heredity is using a <u>Punnett Square</u>.

Explanation:

In genetics, a trait can be considered dominant or recessive. Since the important discoveries of Gregor Mendel, scientists use a <u>capital letter (D) to indicate a trait is dominant, and a lowcase letter (d) to show a trait is recessive. </u>

Assuming that a scientist knows the genotype for the desired trait they want to predict on the offpsring, a Punnett Square is<u> a reliable tool and a graphic representation that permits them to visualize the potential features of the offspring by showing how the alleles may be exhibited and passed on the offspring and will determine the possible combination of genotypes.</u>

<em>How does it work?</em>

For example, as we can observe in the image below, both parents are homozygous for a trait <em>PP=Purple; pp=white</em>. P is dominant and p is recessive (the color purple will be dominant, and the white will be recessive). So, we put one parent's genotype in the top row (PP) and the other parent's genotype in the left column (pp) and combine one of each letters on the squares, <em>i.e</em>. one of each parent's alleles. Then, we can observe in the results that 3 of the offspring will exhibit the purple color (PP, Pp, Pp) and only one will exhibit the white recessive color (pp); or more properly known as a genotypic ratio of 3:1.

3 0
3 years ago
Other questions:
  • How many total polypeptide chains would be present in a single nucleosome core particle?
    11·1 answer
  • The consumer price index measures approximately the same economic phenomenon as
    14·1 answer
  • The outer layer of a cell that acts as a gatekeeper to a cell is the _____.
    8·2 answers
  • Magma that cools and crystallizes on Earth's surface forms A. marble. B. intrusive rocks. C. extrusive rocks. D. quartz.
    13·1 answer
  • List various ways that humans impact the environment and change the ecosystems in which they live. Include your explanation how
    8·2 answers
  • A ---------- is the thing that destroys other things​
    11·1 answer
  • How many types of information are the inthe biology?
    6·1 answer
  • The term demographic transition refers to ________.
    9·1 answer
  • 4. In a species of roundworm, having a hard cuticle (H) is dominant to having a soft cuticle (h). In the lab, a
    5·1 answer
  • 3. Which of the following would best support the theory of evolution?
    6·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!