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enyata [817]
3 years ago
10

Oct4 is an embryonic stem cell specific transcription factor. ESCs grown in petri dishes are fed media that contain basic fibrob

last growth factor (bFGF) which keeps the ESCs undifferentiated. They examine Oct4 mRNA levels in human ESCs (hESCs), hESCs with exogenous miR-223 (micro RNA 223), hESCs with exogenous miR-223 that do not have bFGF in the media, and hESCs without bFGF but with miR-223 inhibitor.Which technique would allow for measurement of Oct4 mRNA levels
Biology
1 answer:
VikaD [51]3 years ago
6 0

Answer:

You can use the quantitative Reverse Transcription Polymerase Chain Reaction PCR (RT-qPCR) method in order to analyze the Oct4 mRNA level.  

Explanation:  

RT-PCR is the gold standard technique to analyze the expression levels of one particular messenger RNA (mRNA) molecule. In this technique, the total RNA is firstly extracted and transcribed into complementary DNA (cDNA). Subsequently, the gene sequence of interest is then amplified in a standard PCR technique that uses as control one or more genes in order to measure the relative expression levels of the target mRNA, in this case, for example, the Oct4 mRNA.

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Flaccid paralysis is a neurological condition characterized by weakness or paralysis and reduced muscle tone without other obvious cause

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Flaccid paralysis causes muscles to contract and go limp. lead to muscle weakness. Spastic paralysis affects tight, rigid muscles. Muscles may twitch or spasm uncontrollably.

Acute flaccid myelitis can be caused by infection with a type of virus known as an enterovirus. Respiratory illness and fever due to enteroviruses are especially common in children. most people recover.

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8 0
2 years ago
Then the atom is pushed up against the other and released, how does it move?
Feliz [49]

Answer:

All matter is made up of atoms and molecules that are constantly moving. When heat is added to a substance, the molecules and atoms vibrate faster. As atoms vibrate faster, the space between atoms increases. ... The atoms can get closer which results in the matter contracting.

Explanation:

8 0
3 years ago
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Nastasia [14]

Answer:

True

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4 0
3 years ago
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Tpy6a [65]

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7 0
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seropon [69]
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3 years ago
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